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What are the advantages of the simple stain technique over the negative stain technique?
Wiki User
∙ 9y ago
Wiki User
∙ 12y ago
Wiki User
∙ 12y agosimple stain is usually when you can just use a wet cloth, or put it in the wash to get it off. but a negative stain is one that never comes off no matter how much you wash it.
Wiki User
∙ 12y agoNegative staining is a technique used to study Bacteria in black back ground .Although stain is used for it , but stain does not stains Bacteria .
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Can you tell an organisms gram stain by simple staining?
Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).
What advantages of negative stain?
Since heat fixation is not required and cells are not subjected to the distorting effects of chemicals and heat, their natural size and shape can be seen. Also, it makes it possible to view hard to stain bacteria such as spirilli.
Is uranyl acetate a negative stain?
Yes, uranyl acetate is used as a negative stain in electron microscopy.
What kind of stain would you use to visualize Sars?
Negative stain
Could a negatively charged dye stain a bacteria?
The effect on the bacteria depends if the stain is an acidic or basic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the bacterial cell, which is slightly negatively charged.
Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the pH is?
8
What is an advantage of a simple stain over a wet mount?
You can see clearer images in the simple stain technique rather than the wet mount technique...
Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
Why is the size more accurate in a negative stain than a simple stain?
since you do not heat fix the slide when you use a negative stain the cells do not shrink or become distorted
Why is the technique called negative staining?
Because the cell wall repels the binding of the negative stain therefore the cells do not stain. Because of this the background is stain with the dye used and the bacteria remain colorless. Basically your staining the background, that is, you are not directly staining the cells.
What bacterial staining technique is used when water is used to remove excess stain from the slide?
simple
What type of microscopy is the negative stain similar to?
The darkfield microscope. "darkfield microscopy"
Can you tell an organisms gram stain by simple staining?
Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).
What is the gram stain for cholera?
Gram Negative
Why doesn't a negative stain colorize the cells in the smear?
A negative stain will stain the background with an acidic dye, such as Nigrosin. This procedure is used to demonstrate capsules. This technique brings the specimen off of the background for more adequate viewing purposes.
Why is size more accurate in an acidic or negative stain than in a simple or basic stain?
Since there is no heat fixing or strong cemicals are used the bacteria are less distorted
What advantages of negative stain?
Since heat fixation is not required and cells are not subjected to the distorting effects of chemicals and heat, their natural size and shape can be seen. Also, it makes it possible to view hard to stain bacteria such as spirilli.
