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Why is the size more accurate in a negative stain than a simple stain?
Wiki User
∙ 16y ago
since you do not heat fix the slide when you use a negative stain the cells do not shrink or become distorted
Wiki User
∙ 16y ago
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Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the PH would be what?
lol, we must be in the same class, b/c i have all of the same questions. did u figure out the answer? I think its a base ... qc the microbiology lab Cute, from the best that I could find some sources say pH 7 and others had given a pH 8. But could not find a solid answer. As a simple stain it would have to have a pH of <7. As we know, the charge on the cell is always going to be negative (an anion). In order to achieve a simple stain we want the stain attracted to the cell wall; therefore, the stain must be positively charged (a cation) for the opposite forces to attract. The more acidic the compound - the smaller its pH number with 7 being neutral. As we know, the [H]+ increases as the pH decreases becoming evermore positively charged. Simple answer: <7
Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
Why doesn't a negative stain colorize the cells in the smear?
A negative stain will stain the background with an acidic dye, such as Nigrosin. This procedure is used to demonstrate capsules. This technique brings the specimen off of the background for more adequate viewing purposes.
What is the purpose of staining an unknown organism?
By doing differential stains on an unknown organism, you can learn more about that organism. One of the most helpful stains would be the Gram stain. The gram stain will differentiate from Gram positive and Gram negative cells, narrowing your bacteria down a lot. Other stains include: Acid-Fast stain, Capsule stain, Endospore stain, and PHB stain.
What is the difference between a simple and a differential stain?
THE DIFFERENTIAL STAIN, SUCH AS THE GRAM STAIN, STAINS THE CELL WALL OF GRAM POSITIVE BACTERIA, DUE TO THE HIGH LEVEL OF PEPTIDOGLYCAN PRESENT IN THE CELL WALL, THESE WILL BE PURPLE IN COLOR. THOSE THAT STAIN GRAM NEGATIVE, DO NOT HAVE AS MUCH PEPTIDOGLYCAN IN THEIR CELL WALL AND WILL RETAIN NEGLIBLE AMOUNTS OF THE CRYSTAL VIOLET AND MORE OF THE SAFFRIN. HENCE THOSE WILL BE GRAM NEGATIVE OR RED TO PINK. THUS THIS TYPE OF STAIN DIFFERENTIATES THE BACTERIA IN TO TWO BASIC CATEGORIES. THIS ASSISTS THE PHYSICIAN IN MAKING A CHOICE REGARDING ANTIBIOTICS TO TREAT THE PATHOGEN WITH. A SIMPLE STAIN JUST SHOWS THE BASIC MORPHOLOGY OF THE BACTERIUM OR BACTERIA PRESENT. YOU MAY WISH TO USE BROMTHYMOL GREEN, CRYSTAL VIOLET... ETC. ALL YOU ARE LOOKING AT IS MORPHOLOGY. HOPE THAT HELPS
Why is size more accurate in an acidic or negative stain than in a simple or basic stain?
Since there is no heat fixing or strong cemicals are used the bacteria are less distorted
Can you tell an organisms gram stain by simple staining?
Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).
Carbolfuchsin can be used as a simple stain and as a negative stain as a simple stain the PH would be what?
lol, we must be in the same class, b/c i have all of the same questions. did u figure out the answer? I think its a base ... qc the microbiology lab Cute, from the best that I could find some sources say pH 7 and others had given a pH 8. But could not find a solid answer. As a simple stain it would have to have a pH of <7. As we know, the charge on the cell is always going to be negative (an anion). In order to achieve a simple stain we want the stain attracted to the cell wall; therefore, the stain must be positively charged (a cation) for the opposite forces to attract. The more acidic the compound - the smaller its pH number with 7 being neutral. As we know, the [H]+ increases as the pH decreases becoming evermore positively charged. Simple answer: <7
Why is the gram stain technique more valuable than the simple stain technique in the diagnostic laboratory Under what circumstances might the simple stain be preferable to the gram stain technique?
There are two types of stains, the simple stain and the differential stain. A simple stain colors all objects the same while a differential stain is used to spot differences in microorganisms. A gram stain is a differential stain, which is used to tell the difference in gram negative and gram positive bacteria. A simple stain would stain all the organisms the same and this difference would not be noted. You would be able to determine their shape, whether it is a cocci or bacillus (rod), but not the type. I'm not sure why the simple stain would be preferable unless you just wanted a quick answer as to the shape of the bacteria. In some cases, a wet prep can be made of a presumptive gram positive cocci to tell the difference between bacteria or yeast. Otherwise, I would say that the gram stain is the only way to go.
Yersinia pestis gram stain?
It is yellow, and blue, but more often yellow though.
Why doesn't a negative stain colorize the cells in the smear?
A negative stain will stain the background with an acidic dye, such as Nigrosin. This procedure is used to demonstrate capsules. This technique brings the specimen off of the background for more adequate viewing purposes.
What is the purpose of staining an unknown organism?
By doing differential stains on an unknown organism, you can learn more about that organism. One of the most helpful stains would be the Gram stain. The gram stain will differentiate from Gram positive and Gram negative cells, narrowing your bacteria down a lot. Other stains include: Acid-Fast stain, Capsule stain, Endospore stain, and PHB stain.
What is one of the most simple ways to make a gun more accurate?
Have it checked by a good gunsmith
Which Gran Positive or negative which is more serious?
Gram positive bacteria responds to the Gram stain; gram negative bacteria does not. The two bacteria do not respond to the same antibiotics. Right now the most dangerous bacteria is a gram negative bacteria. That could change.
How accurate is the UAE exchange rate?
The UAE exchange rate is updated frequently and is considered to be accurate. Be sure to include decimal points in your request; 3.00 will give you a more accurate result than a simple 3.
What is the difference between a simple and a differential stain?
THE DIFFERENTIAL STAIN, SUCH AS THE GRAM STAIN, STAINS THE CELL WALL OF GRAM POSITIVE BACTERIA, DUE TO THE HIGH LEVEL OF PEPTIDOGLYCAN PRESENT IN THE CELL WALL, THESE WILL BE PURPLE IN COLOR. THOSE THAT STAIN GRAM NEGATIVE, DO NOT HAVE AS MUCH PEPTIDOGLYCAN IN THEIR CELL WALL AND WILL RETAIN NEGLIBLE AMOUNTS OF THE CRYSTAL VIOLET AND MORE OF THE SAFFRIN. HENCE THOSE WILL BE GRAM NEGATIVE OR RED TO PINK. THUS THIS TYPE OF STAIN DIFFERENTIATES THE BACTERIA IN TO TWO BASIC CATEGORIES. THIS ASSISTS THE PHYSICIAN IN MAKING A CHOICE REGARDING ANTIBIOTICS TO TREAT THE PATHOGEN WITH. A SIMPLE STAIN JUST SHOWS THE BASIC MORPHOLOGY OF THE BACTERIUM OR BACTERIA PRESENT. YOU MAY WISH TO USE BROMTHYMOL GREEN, CRYSTAL VIOLET... ETC. ALL YOU ARE LOOKING AT IS MORPHOLOGY. HOPE THAT HELPS
Are human cells gram positive?
Human cells are Gram-negative because they do not contain certain structures. More simply, if they don't have a cell wall they cannot be Gram-positive.
