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Medical Technologies

Two small restriction fragment are nearly same size appear as a single band even when the sample is to the very end of the gel what could be donewhy?


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January 21, 2008 2:41AM

Increase the density of the gel. This will slow down the denser fragment while speeding up the less dense fragment. The down side is that the denser fragments near the beginning of the gel near the DNA well will be seen as a single band.