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Two small restriction fragments of nearly the same base pair size appear as a single band even when the sample is run to the very end of the gel What could be done to resolve the fragments?
Wiki User
∙ 12y ago
Increase the density of the gel. This will slow down the denser fragment while speeding up the less dense fragment. The down side is that the denser fragments near the beginning of the gel near the DNA well will be seen as a single band.
Wiki User
∙ 16y ago
Wiki User
∙ 12y agonot too sure, but I'm thinking that you could reverse the polarity for a short while, causing the DNA fragments to move backward and separate the two fragments
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What could be done to resolve two fragments that are nearly the same base-pair length and did not appear on the electrophoresis gel?
You could use other enzymes or use a higher percetage of agarosa to make your gel (so they will have a better chance of separating).
Two small restriction fragments of nearly the same?
Well child, you could increase the density of the gel and that would allow for the smaller of the two fragments to move farther, but keep the larger one of the two from moving just as far.
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