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Can i use saline citrate buffer for DNA extraction from tissue of bivalve?
yes??
Why we use two washing buffers in DNA extraction using biosprint?
the washing buffers contain ethanol which precipitating DNA molecules and form clumps. DNA is insoluble in alcohol so come together in an alcohol buffer.
How do you store DNA extraction buffer containing PVP?
hi, I work with CTAB extraction and I've noticed that is better to make the buffer without PVP and only add the PVP when you need the buffer. So I take 20ml CTAB and add 0.8mg PVP. Mix it and I use it not longer than 1 week. Hope this helps
Why you are using SE buffer in DNA extraction?
TE buffer protect DNA or RNA from degradation. "TE" is derived from its components: Tris (Interact with the lipopolysaccharide and lyes the cell membrane and prevent other cells from attacking), and EDTA, a molecule chelating agent. It is commonly use to protect the DNA or RNA while storing it.
Why is DNA wash buffer used in DNA extraction?
It helps break the nuclear membrane of the cell. Detergent containing the compound SDS ( sodiumdodecyl sulfate) is used to break down and emulsify the fat and proteins that make up a cell membrane.
Can i use saline citrate buffer for DNA extraction from tissue of bivalve?
yes??
Why you use ammonium chloride and ammonium carbonate in lysis buffer for DNA extraction from blood?
In lysis buffer, the function of ammonium chloride is to determine the red blood cells in samples. These samples also contain white blood cells.
What is Buffer ATL?
Buffer ATL is a proprietary chemical used for DNA extraction and purification. It's used to induce lysis of cell tissues in order to release and expose the cell's DNA during the beginning stages of of the extraction process. I think the letters "ATL" stand for "Animal Tissue Lysis." It's part of the DNeasy protocol from Qiagen. I use this every day during my extraction and purification of DNA from various snake species.
Why we use two washing buffers in DNA extraction using biosprint?
the washing buffers contain ethanol which precipitating DNA molecules and form clumps. DNA is insoluble in alcohol so come together in an alcohol buffer.
How do you store DNA extraction buffer containing PVP?
hi, I work with CTAB extraction and I've noticed that is better to make the buffer without PVP and only add the PVP when you need the buffer. So I take 20ml CTAB and add 0.8mg PVP. Mix it and I use it not longer than 1 week. Hope this helps
Why you are using SE buffer in DNA extraction?
TE buffer protect DNA or RNA from degradation. "TE" is derived from its components: Tris (Interact with the lipopolysaccharide and lyes the cell membrane and prevent other cells from attacking), and EDTA, a molecule chelating agent. It is commonly use to protect the DNA or RNA while storing it.
Could you use a piece of liver in DNA extraction?
yes
What is the function of ammonium acetate in DNA extraction?
ammonium acetae use to percipitate DNA from water.
Why is DNA wash buffer used in DNA extraction?
It helps break the nuclear membrane of the cell. Detergent containing the compound SDS ( sodiumdodecyl sulfate) is used to break down and emulsify the fat and proteins that make up a cell membrane.
Why use wbc instead of rbc in DNA extraction?
Because they dont have a nucleous.
What is the point of mashing straberrys for DNA extraction?
Use the usual extraction protocol for plants: grind, liquid-liquid extraction, salting out.
What we use for delete DNA extraction sample color?
one of the matherials that we use for this is cloroform. others...
