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hi,
I work with CTAB extraction and I've noticed that is better to make the buffer without PVP and only add the PVP when you need the buffer.
So I take 20ml CTAB and add 0.8mg PVP. Mix it and I use it not longer than 1 week.
Hope this helps
Wiki User
∙ 15y ago
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Why extraction buffer is added for isolation of DNA?
Extraction Buffer is used to maintain pH of the solution.which prevents denaturation of DNA.
What is function of Buffer AP1 in DNA extraction?
The buffer AP1 is vital in DNA extraction as it acts as a cleanser to break up the lipids surrounding the cellular membrane. The buffer also maintains the right environment for the DNA so it is not damaged during the extraction process.
Can i use saline citrate buffer for DNA extraction from tissue of bivalve?
yes??
Why is buffer HB used in DNA extraction?
It serves to break the tissue apart so the DNA can be subsequently extracted.
What is content se- buffer in DNA extraction?
Tris pH 8.0 NaCl EDTA
What is the function of CL buffer in DNA extraction?
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
Why extraction buffer is added for isolation of DNA?
Extraction Buffer is used to maintain pH of the solution.which prevents denaturation of DNA.
What is function of Buffer AP1 in DNA extraction?
The buffer AP1 is vital in DNA extraction as it acts as a cleanser to break up the lipids surrounding the cellular membrane. The buffer also maintains the right environment for the DNA so it is not damaged during the extraction process.
What is the function of tritonx100 in DNA extraction?
Triton X-100 is used as a lysis buffer for DNA separation.
Can i use saline citrate buffer for DNA extraction from tissue of bivalve?
yes??
Why was detergent added to extraction buffer?
because it can break through the membranes to get to the DNA
Why is buffer HB used in DNA extraction?
It serves to break the tissue apart so the DNA can be subsequently extracted.
What is content se- buffer in DNA extraction?
Tris pH 8.0 NaCl EDTA
What is the purpose of NACL is DNA extraction?
Sodium chloride help the separation of DNA from other proteins.
What is the purpose of using soap in a DNA extraction buffer?
The word detergent is used instead of soap in a DNA extraction buffer. Detergent is used to create a hydrophobic environment that favors the precipitation of proteins. Proteins are one of two major contaminants in DNA extraction (the other major contaminant being RNA). When protein precipitate, they can be separated by centrifugation and the DNA isolation procedure can continue.
Why you use ammonium chloride and ammonium carbonate in lysis buffer for DNA extraction from blood?
In lysis buffer, the function of ammonium chloride is to determine the red blood cells in samples. These samples also contain white blood cells.
Why is DNA wash buffer used in DNA extraction?
It helps break the nuclear membrane of the cell. Detergent containing the compound SDS ( sodiumdodecyl sulfate) is used to break down and emulsify the fat and proteins that make up a cell membrane.
