what type of leaf?
When you boil a leaf in ethanol, the ethanol will extract the pigments and other compounds from the leaf. The chlorophyll will dissolve in the ethanol, causing the leaf to lose its green color and appear pale. The ethanol will take on the color of the extracted pigments, turning it into a green solution.
Well, honey, before boiling, that leaf is as rough as a cat's tongue on a bad hair day. It's got bumps and ridges, probably more texture than your last Tinder date. So, if you're looking for something smooth and silky, you better toss that leaf in some hot water and let it simmer.
the green pigment, chlorophyll should be removed in order to observe color change during test for starch. in order to do that, the leaf is dipped in boiling water to break cell walls which would facilitate the release of the pigment out of the cell. immersion in ethanol would dissolve the pigment which would be released out of the cells thereby making the leaf appear pale in color.
After being soaked in ethanol, a leaf may appear discolored or faded with a translucent appearance. The waxy cuticle coating on the leaf surface may have been removed, giving the leaf a more pliable texture.
When a leaf is heated by ethanol, it may become wilted, dried out, discolored, or charred depending on the temperature and duration of heating. The heat from the ethanol can cause the leaf's water content to evaporate, leading to changes in its appearance and texture.
add the leaf to boiling ethanol in a water bath for a few minutes (the boiling ethanol dissolves the chlorophyll and removes the green colour from the leaf - it turns white so it is easy to see the change in colour) wash with water to rehydrate and soften the leaf
You can remove the crispy leaf by gently lifting it out of the ethanol solution using tweezers or forceps. Be cautious not to damage the leaf during the removal process as it may be fragile after the boiling.
Boiling in ethanol strips the leaf's waxy outer coating off, then the iodine turns it blue black because it's reacting to the presence of starch, a product of photosynthesis.
Half fill a beaker with boiling water and add a large test tube that is a quarter full of ethanol. Allow the ethanol to come to a boil. Do not heat the ethanol in a Bunsen burner flame. This is not safe because ethanol is highly flammable. Take a leaf that has been sitting in good light for at least a few days, and soften in the boiling water for ten seconds or so. Then add to the ethanol, and allow to boil for about a minute until all the color disappears from the leaf. Remove the leaf from the ethanol. Put it back in the hot water to soften for 10 seconds. Spread the leaf out on a white tile and use the iodine solution to test for starch a blue-black color indicates starch is present.
A plant is destarched by placing a leaf under investigation in boiling water to get rid of all the excess starch. Then place into boiling ethanol to get rid of excess chlorophyll
Boiling the leaf in ethanol removes chlorophyll, which can interfere with the test for starch using iodine. This process helps to ensure accurate detection of starch in the leaf tissue without any color interference from chlorophyll.
Rose leaf has smooth texture