Ethanol dissolves chlorophyll hence further phtosynthetic activity is stopped in the abscence of light and the leaf becomes transparent (colorless). the colorless leaf takes better stain with iodene while testing for the presence of starch.
A hot bath of ethanol decolorizes the leaf by washing out the chlorophyll. If the leaf is not decolorized, you cannot see the blue-black stain that results from the iodine reacting with the starch.
Covering a leaf with iodine helps to test for the presence of starch. Iodine reacts with starch to produce a blue-black color, indicating the presence of this carbohydrate in the leaf. This test is commonly used in biology experiments to detect the storage of starch in plant leaves.
If the iodine solution stays orange after testing a leaf for starch, it indicates that the leaf does not contain starch. Starch would typically turn the iodine solution blue-black in color. Therefore, the orange color suggests that the leaf did not produce a significant amount of starch through photosynthesis.
Rinsing a leaf in cold water after boiling it is done to stop the enzymatic activity that breaks down starch. Boiling the leaf softens the cell walls, allowing the iodine solution to penetrate and react with any starch present. Rinsing in cold water halts the enzymatic breakdown of starch, preserving the blue-black color reaction with iodine, indicating the presence of starch in the leaf.
Boiling a leaf for a starch test is done to remove any pigments or chlorophyll present in the leaf that could interfere with the test results. The heat breaks down the cell membranes and denatures enzymes that could potentially affect the starch test. Boiling also helps to soften the leaf tissue, making it easier to extract the starch for testing purposes.
to denature the enzymes going to kill the leaf
The leaf turns brittle during the testing the leaf for starch because the ethanol extracts the all water content from the leaf.
When testing for starch, the leaf is boiled in alcohol to remove the chlorophyll that may mask the iodine-starch color change. If the leaf turns blue-black after applying iodine, it indicates the presence of starch. If the leaf remains discolored, it suggests that starch is absent, as the iodine did not react with any starch molecules present in the leaf.
The iodine test is commonly used to detect the presence of starch in a sample. If the leaf turns blue-black when treated with iodine solution after boiling with ethanol, it indicates the presence of starch in the leaf tissue.
Boiling in ethanol strips the leaf's waxy outer coating off, then the iodine turns it blue black because it's reacting to the presence of starch, a product of photosynthesis.
A hot bath of ethanol decolorizes the leaf by washing out the chlorophyll. If the leaf is not decolorized, you cannot see the blue-black stain that results from the iodine reacting with the starch.
The leaf is boiled in alcohol to remove the chlorophyll and other pigments that can interfere with the starch test. This process helps to showcase the presence of starch in the leaf by removing any substances that may obscure the results.
add the leaf to boiling ethanol in a water bath for a few minutes (the boiling ethanol dissolves the chlorophyll and removes the green colour from the leaf - it turns white so it is easy to see the change in colour) wash with water to rehydrate and soften the leaf
Covering a leaf with iodine helps to test for the presence of starch. Iodine reacts with starch to produce a blue-black color, indicating the presence of this carbohydrate in the leaf. This test is commonly used in biology experiments to detect the storage of starch in plant leaves.
Use iodine to test a leaf for starch | Plant Physiology | Biology
Half fill a beaker with boiling water and add a large test tube that is a quarter full of ethanol. Allow the ethanol to come to a boil. Do not heat the ethanol in a Bunsen burner flame. This is not safe because ethanol is highly flammable. Take a leaf that has been sitting in good light for at least a few days, and soften in the boiling water for ten seconds or so. Then add to the ethanol, and allow to boil for about a minute until all the color disappears from the leaf. Remove the leaf from the ethanol. Put it back in the hot water to soften for 10 seconds. Spread the leaf out on a white tile and use the iodine solution to test for starch a blue-black color indicates starch is present.
so the waxy cuticles can rub off the surface of the leaf. a leaf has a cell wall and if you did not put the leaf in the boiling water it would not break down so therefore you would not be able to do a proper starch test on the leaf so the answer to this question is to break the cell wall down so you can test for starch properly i hope i helped you :) yeap