add the leaf to boiling ethanol in a water bath for a few minutes (the boiling ethanol dissolves the chlorophyll and removes the green colour from the leaf - it turns white so it is easy to see the change in colour) wash with water to rehydrate and soften the leaf
leaf is boiled in alcohol so that the green pigment that's chlorophyll is removed which lightens the green colour of the leaf.see the lab manual of class 10 (NCERT) u have this experiment in it.
It is boiled in ethanol so it can remove the chlorophyll.
So then the ethanol could extract the chlorophyll from the leaf
because the chlorophyll is green and it stained the etanol
by immersing it in a hot water bath
the ethanol will change colour and the leaf will soften
our eyes detect the green light that is reflected off the leaf
Starch, I think, because iodine solution is the test for starch.
what colour does the clear alcohol become once the leaf is boiled in it
Ethanol extracts chlorophyll from leaf .
Ethanol dissolves chlorophyll hence further phtosynthetic activity is stopped in the abscence of light and the leaf becomes transparent (colorless). the colorless leaf takes better stain with iodene while testing for the presence of starch.
by immersing it in a hot water bath
it will make holes
The cells all burst and the leaf looses turgidity = the leaf dies
After the leaf has been placed in ethanol it becomes very brittle. It is then rinsed in cold water so as to soften it and makes it more manageable for the addition of iodine.
the ethanol will change colour and the leaf will soften
Boiling in ethanol strips the leaf's waxy outer coating off, then the iodine turns it blue black because it's reacting to the presence of starch, a product of photosynthesis.
our eyes detect the green light that is reflected off the leaf
Light that is white contains all the colours. When the light hits the leaf, it absorbs all the colours apart from the green light which is reflected back out to your eyes. So you see the leaf as green.
Starch, I think, because iodine solution is the test for starch.
it reflects green light