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What color would the bacterial cell have when viewed microscopically after the treatment with ammonium oxalate crystal violet?
Violet
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
Function of Gram's iodine?
Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.
Why do you add crystal violet stain in simple staining method?
Because violet dye is the least expensive.
What happen if you forget to add crystal violet to a stain?
You don't get a counterstain.
What color would the bacterial cell have when viewed microscopically after the treatment with ammonium oxalate crystal violet?
Violet
What is crystal violet iodine complex?
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
What is gentian violet?
Traditional antifungal drugs such as.gentian violet (also known as crystal violet)
Explain why crystal violet would not be useful in the negative stain technique?
Negative staining techniques are designed to stain everything BUT the bacterial cells. This allows us to see the cells unstained and helps us observe their morphology (how the cells are shaped and how they group together). The cells have an overall negative charge on their surface, so they naturally attract positive charges. Crystal Violet carries a positive charge, so it would adhere to the surfaces of the cells thus staining the cells (which is not what you want in a negative stain!)
What is the extinction coefficient of crystal violet?
balls
Function of Gram's iodine?
Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.
Why is Iodine used in Gram staining?
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
Crystal violet acid-base indicator?
Yes it is.
Function of crystal violet?
Crystal violet is a hexamethyl also known as methyl violet 10B This is much darker than 2B, and often darker than 6B. It is used in biological stainang particularly gram staining together with safranin and iodine. Crystal violet was also used to treat strept throat. Doctors used to swab it on the back of your throat.
Why do you add crystal violet stain in simple staining method?
Because violet dye is the least expensive.
