Asked in Biology, Chemistry, Microbiology, Biotechnology
What is shake flask fermentation?
Shake Flask fermentation is a cheapest ans simplest technique to grow bacteria or fungi, aerobically, in small volumes of nutrient broth. The broth is poured into erlenmeyer flasks equipped with cotton-wool stoppers, and autoclaved. After cooling, some microbes are "seeded" into the flask, and it is placed on a Shaker machine. The shaking agitates the content and so ensures aeration, so that the microbes could breathe. ...
Asked in Chemistry
What solution to rinse volumetric flask with?
Rinse the volumetric flask twice with the solution that you about to fill it with, stopper the opening and shake the flask so that all surface area inside the flask will be covered. This is so that the solution will not be diluted or contaminated when poured into the flask. ...
Asked in Microbiology, Scientists, Louis Pasteur
How did Louis Pasteur disprove the spontaneous generation theory?
Louis broiled broth for 15 min. He then let it sit for one year in a swan neck flask. He noticed that some dust had accumulated in the top part of the flask. So he took a sample, form the broth inside the flask. He noticed that nothing was inside of growing in it. So he then preceded to shake the broth in the flask in such a way, that it would touch the dust at the top. He then...
Asked in Elements and Compounds
What happens when you mix red phosphorus Iodine crystals and ephedrine?
You get meth in about 40 minutes. Throw it all on one flask and let it sit for a while shake the flask it if starts to bubble up too much. Then when it calms down heat it to about 180f until it doesnt look like anything else is happening. ...
Asked in Rock Music
What is every song that has the words Shake shake shake shake it in it?
i only know one song and that is of cours Shake It by metro station it has the lyrics shake shake shake shake shake it there's also the song by outkast shake it like a polaroid picture .... i believe it's called hey ya ...
Asked in Chemistry
How do you clean a volumetric flask?
Acid washing it should do. I assume you are in a lab...in which case a 1:1 Hydrochloric Acid solution can be poured into the flask. DON'T FORGET TO PUT GOGGLES AND GLOVES ON. Put the lid on. Shake the flask and turn it to ensure all surfaces of the glass inside are being washed with the acid. Then, give it a good triple rinse with de-ionized water. If you are just at home, pour in some white vinegar and let it sit...
Asked in The Difference Between
What is the difference between primary and secondary screening of microorganisms?
primary screening is a solid agar plate assay method and is a qualitative method after which secondary screening is done,in which the microbes are screened in a liquid shake flask condition and assessed quantitatively. ...
Asked in Shake It Up (TV Series)
What song has the lyrics shake it up boom shake it shake it up shake it up boom shake it up earthquake?
Shake it up how can I get on the show im a good dancer please let me on the show ...
Asked in Milkshakes and Malts
How much milk could a milkshake shake if a milkshake could shake milk?
If a milkshake could shake milk, a milkshake would shake all the milk that a milkshake could shake. ...
Asked in Animal Life
What are the four essential aspects of successful training?
1: Food, praise, stimulation, exercise 2:leash,collar, choke chain, shake can 3:Instinct, conditioning, education, observation 4: reward, reprimand, trust, consistency The four essential aspects of successful training are first know the product that your are trained on, learn the different aspects, learn where failures appear, and conquer any discretion . ...
Asked in Parts of Speech, Verbs
Is shake an action verb?
Yes, the verb 'shake' is an action verb, a word for an action. Example: I'd like to shake your hand. The word 'shake' is also a noun. Example: The shake was felt hundreds of miles from the epicenter. Verb: shake, shakes, shaking, shaken, shook. Noun: shake, shakes. ...
Asked in Chemistry
What are all the different types of flasks?
Laboratory Glassware: Types of Laboratory Flasks Flasks are useful tools for containing liquid and performing mixing, heating, cooling, precipitation, condensation and other processes. They come in a range of sizes, materials and uses. Commonly used laboratory flasks include: An Erlenmeyer flask, conical, or E-flask is one of the most commonly used flasks in scientific laboratories. The flask has a conical base that almost extends into a small, cylindrical neck. This shape allows the flask to be sealed with a bung for heating purposes, while...