500 ml
Shake Flask fermentation is a cheapest ans simplest technique to grow bacteria or fungi, aerobically, in small volumes of nutrient broth. The broth is poured into erlenmeyer flasks equipped with cotton-wool stoppers, and autoclaved. After cooling, some microbes are "seeded" into the flask, and it is placed on a Shaker machine. The shaking agitates the content and so ensures aeration, so that the microbes could breathe.
To make a 9 grams per liter solution of NaCl, simply dissolve 9 grams of NaCl in enough water to make a total volume of 1 liter. Stir or shake the solution until the NaCl is completely dissolved. Measure the volume accurately using a graduated cylinder or volumetric flask.
Acid washing it should do. I assume you are in a lab...in which case a 1:1 Hydrochloric Acid solution can be poured into the flask. DON'T FORGET TO PUT GOGGLES AND GLOVES ON. Put the lid on. Shake the flask and turn it to ensure all surfaces of the glass inside are being washed with the acid. Then, give it a good triple rinse with de-ionized water. If you are just at home, pour in some white vinegar and let it sit for a while. That should break up anything that's inside.
Louis broiled broth for 15 min. He then let it sit for one year in a swan neck flask. He noticed that some dust had accumulated in the top part of the flask. So he took a sample, form the broth inside the flask. He noticed that nothing was inside of growing in it. So he then preceded to shake the broth in the flask in such a way, that it would touch the dust at the top. He then took a sample from that and it was highly bacteria ridden.
to mix the ingredients..................but i don't shake it................
Shake flasks, such as the Erlenmeyer flask, are designed to prevent splashing. They promote oxygen transfer and prevent vibration, allowing for successful fermentation.
so that the solutions mix properly
Shake Flask fermentation is a cheapest ans simplest technique to grow bacteria or fungi, aerobically, in small volumes of nutrient broth. The broth is poured into erlenmeyer flasks equipped with cotton-wool stoppers, and autoclaved. After cooling, some microbes are "seeded" into the flask, and it is placed on a Shaker machine. The shaking agitates the content and so ensures aeration, so that the microbes could breathe.
A couple of benefits of working at Steak & Shake is a stable job and earning money.
The song I'm pretty sure you are talking about that goes "shake ya a** watch yourself shake ya a** show me what you're working with" is shake ya a** by mystikal
Drink a protein shake.
To make a 9 grams per liter solution of NaCl, simply dissolve 9 grams of NaCl in enough water to make a total volume of 1 liter. Stir or shake the solution until the NaCl is completely dissolved. Measure the volume accurately using a graduated cylinder or volumetric flask.
No duce is not dead he is still working with cece and rocky on shake it up
To drink your milk shake
he does butt clenches about 10 each day and a 500ml protein shake .......seriously get a life OK that wasn't really nice and I was just wondering out of random
if you take your booty and shake it you can find out
Acid washing it should do. I assume you are in a lab...in which case a 1:1 Hydrochloric Acid solution can be poured into the flask. DON'T FORGET TO PUT GOGGLES AND GLOVES ON. Put the lid on. Shake the flask and turn it to ensure all surfaces of the glass inside are being washed with the acid. Then, give it a good triple rinse with de-ionized water. If you are just at home, pour in some white vinegar and let it sit for a while. That should break up anything that's inside.