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Restriction enzymes (also known as restriction endonucleases) are proteins which cut DNA up at specific sequences in the genome. For example, the commonly used restriction endonuclease EcoRI recognizes every point in DNA with the sequence GAATTC, and cuts at the point between the Guanine and Adenine. Interestingly, the recognition sequences for most restriction endonucleases are genetic palindromes, e.g., the sequence reads exactly the same backwards on the complementary strand. In the case of EcoRI, the two complementary DNA strands for the recognition sequence are: 5'--GAATTC ---3'3'--CTTAAG--5'
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∙ 12y ago
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∙ 9y agoRestriction endonucleases are the enzymes that cut nucleic acids like DNA. Many endonuclease are sequence specific. The specific sequence of nucleotides on the DNA that the endonuclease recognizes and binds to in order to make a cut is called the recognition site.
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∙ 14y agoThe restriction enzyme cleavage site is a specific site on the DNA strand that has a defined sequence of nucleotides. The restriction enzyme recognizes this sequence, binds to the DNA and produces a cut.
For example, for the restriction enzyme EcoR1, the restriction site is GAATCC. Therefore, whenever EcoR1 finds GAATCC, it binds to the DNA at that location and produces a cut
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∙ 12y agothose place where restriction enzyme cut the DNA molecules
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∙ 13y agoRestriction Enzymes: DNA cutting enzymes found in bacteria. These enzymes recognize and cut DNA at particular sequences of nucleotides (called their recognition sites).
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∙ 10y agodouble-stranded DNA
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What enzyme do scientists use to cut genes out of strands if DNA?
restriction enzymes
Do restriction enzymes cut protein molecules at specific sites?
No. restriction enzymes do not cut proteins. restriction enzymes cut DNA molecules at specific sites called restriction sites.
Which of the following cuts DNA molecules at specific locations?
restriction endonuclease enzymes
What does a geneticist use to cut DNA at specific base sequences?
Restriction enzymes, also known as restriction endonucleases, are used to cut DNA into smaller fragments. Restriction enzymes are found in bacteria, where they act like molecular scissors by cutting up DNA from invading viruses or bacteriophages. Each restriction enzyme recognizes a specific nucleotide sequence and cuts the DNA at that site. This process makes restriction enzymes extremely useful in biotechnology where they are used in procedures such as DNA cloning, DNA fingerprinting, and genetic engineering. There are hundreds of known restriction enzymes, and each one was named for the bacteria from which it was isolated. For example, EcoRI was isolated from Escherichia coli and HaeIII from Haemophilus aegyptius.
Are bacteria a source of restriction enzymes in DNA?
Restriction enzymes are made by the ribosomes in bacteria. For information on which bacteria produce which specific restriction enzymes, some can be found in a table near the bottom of this page: http://en.wikipedia.org/wiki/Restriction_enzymes
What enzyme do scientists use to cut genes out of strands if DNA?
restriction enzymes
Which pair of enzymes is necessary to make recombinant DNA?
Actually the answer would be Restriction enzyme and DNA ligase.
Enzymes used to cut DNA molecules in recombinant DNA researsh are?
restriction enzymes
Do restriction enzymes cut protein molecules at specific sites?
No. restriction enzymes do not cut proteins. restriction enzymes cut DNA molecules at specific sites called restriction sites.
How are large DNA molecules cut up?
by using restriction enzymes
What are enzymes cutting DNA at specific sites to form restriction fragments called?
restriction enzymes or endonuclease enzymes
What is used to cut the DNA chain so that new genes may be inserted?
Restriction Enzymes
Which of the following cuts DNA molecules at specific locations?
restriction endonuclease enzymes
What organism uses restriction enzymes?
Bacterias use restriction enzymes as a form of defense mechanism. We as people use these restriction enzymes in bacterias to aid us in genetic engineering.
What does a geneticist use to cut DNA at specific base sequences?
Restriction enzymes, also known as restriction endonucleases, are used to cut DNA into smaller fragments. Restriction enzymes are found in bacteria, where they act like molecular scissors by cutting up DNA from invading viruses or bacteriophages. Each restriction enzyme recognizes a specific nucleotide sequence and cuts the DNA at that site. This process makes restriction enzymes extremely useful in biotechnology where they are used in procedures such as DNA cloning, DNA fingerprinting, and genetic engineering. There are hundreds of known restriction enzymes, and each one was named for the bacteria from which it was isolated. For example, EcoRI was isolated from Escherichia coli and HaeIII from Haemophilus aegyptius.
Are bacteria a source of restriction enzymes in DNA?
Restriction enzymes are made by the ribosomes in bacteria. For information on which bacteria produce which specific restriction enzymes, some can be found in a table near the bottom of this page: http://en.wikipedia.org/wiki/Restriction_enzymes
Which enzyme opens DNA molecules?
Restriction Enzymes
