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A technique that exploits variations in homologous DNA sequences
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Differences in DNA sequence on homologous chromosomes that can result in different patterns of restriction fragment lengths?
Restriction fragment length polymorphism (RFLP)
What type of enzyme is used to fragment dna?
restriction enzymes are used to cut DNA.
How do you detect DNA?
DNA can be detected by a modern process called southern blotting
Single stranded pieces of DNA left on both margins of a ristriction fragment of DNA that has been cut with a restriction enzyemare called?
sticky ends
What is another name for DNA strand?
DNA profiling and DNA typing. A more technological term used in laboratories is RFLP analysis, or Restriction Fragment Length Polymorphism.
Differences in DNA sequence on homologous chromosomes that can result in different patterns of restriction fragment lengths?
Restriction fragment length polymorphism (RFLP)
What is the rflps?
Do you mean "RFLP" if so its, restriction fragment length polymorphism. (DNA analysis)
DNA moleule with length1800bp were treated with cutting enzyme Hae3 and two fragments were produced one a length of 1000bp so how many restricton sites for Hae3 exist inthis DNA and the length of seco?
the restriction endonuclease(HaeIII) has one restriction site on that DNA molecule. If one of the fragment is 1000bp long then the length of other fragment should be 800bp.
Why do the number of DNA fragments and the length of each fragment produced by RFLP analysis differ from person to person?
As the DNA fragments results from the action of the restriction enzymes and on the other hand mutations alter the sites where the restriction enzymes react therefore there is difference in number and of length of each fragment from person to person.
What method is used to make DNA fingerprints?
A method known as RFLP (restriction fragment length polymorphism) analysis can be used to make a DNA fingerprint.
What type of enzyme is used to fragment dna?
restriction enzymes are used to cut DNA.
How are DNA from hair follicle and skin cell used to determine if they belong to same person?
DNA can be extracted from tissue, in this case, epidermal layers of skin (keratin doesn't have nuclei) or the hair follicle. The DNA is then amplified using a process called polymerase chain reaction (PCR), to create many more copies of the DNA segments using special coding sequences called primers. The segments can then be digested with enzymes (restriction enzymes) to create DNA fragments. Differences in DNA sequences create different sized DNA fragments, which are then separated on a gel matrix using an electric current (electrophoresis). The pattern of different sized fragments is unique to an individual, somewhat like a bar code. Two similar fragment patterns are compared, and if similar suggest identity (RFLP - restriction fragment length polymorphisms). You increase the chances of proving identity between the hair and skin if many different restriction enzyme reactions are done showing similarity between many different fragment sizes.
How do you detect DNA?
DNA can be detected by a modern process called southern blotting
How many restriction enzymes are typically needed to positively identify a sample of DNA?
It usually takes 10 to 12 different restriction enzymes to produce a fragment pattern. This will allow positive identification of an individual.
What is RFLP used for?
RFLP (often pronounced "rif lip", as if it were a word) is a method used by molecular biologists to follow a particular sequence of DNA as it is passed on to other cells. RFLPs can be used in many different settings to accomplish different objectives. RFLPs can be used in paternity cases or criminal cases to determine the source of a DNA sample. RFLPs can be used determine the disease status of an individual. http://www.bio.davidson.edu/COURSES/genomics/method/RFLP.html
Which fragment of DNA is the longest or biggest?
The fragment of the DNA that is the longest is the one that is the slowest to get to the bottom of the gel in the body. This is because longer DNA are simply the largest base pairs that are digested in the restriction enzymes which make them slower then the shorter ones.
In molecular biology RFLP is an acronym for what?
RLFP is an acronym for Resriction Fragment Length Polymorphism. RLFP analysis is used to identify changes in a genetic sequence that occurs at a site where a restriction enzyme cuts. RFLP's can be used to identify specific mutations and also trace inheritance patterns!
