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it is not the acid ether concentration technique which produces four layers, but the formalin-ether concentration technique, which, at the end of the procedure after centrifugation will produce an upper layer of ether, a second layer of fat debris, a third layer of formalin and the bottom layer of sediments which contain your eggs and cysts...

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Q: What are the 4 layers obtained in the acid ether concentration technique?
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