Liquid culture: bacterial cells are places in sterile liquid culture medium and placed in a shaking incubator at the advised temperature
On culture plates: Bacteria are plated (or streaked) on to media placed in culture plates. These plates are held in an incubator at the recommended temperature
You can use the Candle Jar method, the GasPak System and there's another one but I can't think of it at the moment.
thanks
Culture
VAS (vaseline) PAR (paraffin) - Is a 50/50 mix used to seal the air out of a microbiological tube culture/test, making it an anaerobic environment.
TSA plates do not allow for the growth of anaerobes, becasue the entire culture is exposed to oxygen. So the TSA plates require the gaspak to provide an anaerobic condition. FTM does allow for the growth of anaerobes and aerobes. Anaerobes will be found deep in the soln. away from the oxygen, and aerobes will be found at the top, with facultatives inbetween.
cotton fibers may be detrimental to anaerobes. Abscesses or fluids can be aspirated using a sterile syringe that is then tightly capped to prevent entry of air.
Cultures look for bacterial growth. And thus in order for bacteria to grow in a given culture medium LIVE bacteria are needed. Depending on the mechanism for obtaining a sample, and the sample placed in the culture medium (i.e. swab, probe, biopsy, blood, etc.) the probability of obtaining live bacteria varies. Pus that usually accompanies an infection is composed of dead bacteria within white blood cells. And thus the swab of a skin wound full of pus is UNlikely to yield a positive (defined by bacterial growth in the culture medium) result. In short live bacteria is needed for a culture to be reported as positive. Not all samples taken for culture contain live bacteria, even in the presence of an infection (it is just the live bacteria are too deep to be accessed by the sampling technique used. I hope this answers your question. Sincerely, Matthew C. Lee, MD, RPh, MS
It may depend on the brand of blood culture bottles, but there are generally 2 types of bottles drawn on an adult patient; one to culture aerobic organisms and one for anaerobic or facultatively anaerobic organisms. Anaerobic organisms require no oxygen for growth. Facultative means the organism seems to do better under anaerobic conditions. AN for anaerobic...perhaps the F means facultatively.
an aerobic culture is the one which requires oxygen for the growth of microbes
a method used to grow anaerobes from a clinical specimen.
Aerobic needs oxygen to grow.
ARD stands for aerobic/anaerobic blood culture bottles, which refers to the type of bottles used to collect blood samples for culture. Aerobic bottles allow for the growth of bacteria in the presence of oxygen, while anaerobic bottles allow for the growth of bacteria in the absence of oxygen.
Proteus can grow in anaerobic conditions due to the fact that it is a facultative anaerobe. It can also, however, grow in oxygenated conditions for the same reason.
describe the "culture of consumption" during the Eisenhower Adminstration
A constantly changing culture could be described as dynamic, evolving, or fluid. It may also be characterized as adaptive or progressive.
describe the work environment or culture in which you are most productive and happy
The oldest in the world.
Culture
tradition