The major drawback is that treatment with SDS denatures the protein, meaning you are not looking at it in its natural state.
tris acting as a buffer to control the pH of the solution--abu
TEMED is used during the gel preparation for SDS PAGE. It initiates polymerization of ammonium per sulphate. Hence if you add more TEMED the gel becomes hard and if you add less than required the gel does not solidifies!
Polymerization of acrylamide and bisacrylamide monomers is induced by ammonium persulfate (APS), which spontaneously decomposes to form free radicals
SDS or sodiumdodecyl sulfate is a detergent used in protein separation. SDS buffer or SDS sample buffer consist of SDS, Tris, glycerol, bromo phenol blue, EDTA, and DTT or beta mercapto ethanol as a standard recipe. SDS is also added in stacking and separating gel preparation buffers that contain acrlamide.The main purpose is to keep the proteins denatured and provide the net negative charge to proteins as well as to run them according to it molecular weight
SDS (Sodium Dodecyl Sulfate or [CH3-(CH2)10-CH2-O-SO-3]Na+) is a detergent under the category of amphipathic molecules (those molecules that have both polar or hydrophilic, and nonpolar or hydrophobic groups) and is a strong denaturant chemical that disrupts the terciary structure of proteins (without breaking disulfide bonds) to give a random coil or rod-like shape configuration.SDS binds quite tenaciously to proteins (an average of one SDS molecule for every two amino acid residues). The large negative charge that SDS imparts masks the protein's intrinsic charge so that SDS-treated proteins tend to have identical charge-to-mass ratios and similar shapes, useful characteristics to determine their molecular masses through SDS-PAGE electrophoresis techniques.
may be because of toomany disulfide linkages
glycine molecular weight high so mobility also high so using in SDS PAGE
Due to many proline residues it migrates slower on sds page and appears heavier than it is.
It is the gel of choice for SDS PAGE
Laemmli U. K.
SDS-PAGE method
Glycine increases the mobility of the gel.
Electrophoresis is the method that could be used to further separate two bands from the same protein fraction after SDS-PAGE.
break the S-S bonds in a protein
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SDS-PAGE AGAROSE CAPILLARY SEQUENCING TO NAME A FEW
SDS - PAGE is apparently used to seperate proteins. The proteins are by nature different sizes. SDS works as a stabilizer by separating proteins according to similar forms.