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Due to many proline residues it migrates slower on sds page and appears heavier than it is.
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∙ 12y ago
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What type of gene is the p53 gene?
p53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53gene. p53 is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressorthat is involved in preventing cancer. As such, p53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation.The name p53 is in reference to its apparent molecular mass: It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. But, based on calculations from its amino acid residues, p53's mass is actually only 43.7 kDa. This difference is due to the high number of prolineresidues in the protein, which slows its migration on SDS-PAGE, thus making it appear heavier than it actually is. This effect is observed with p53 from a variety of species, including humans, rodents, frogs, and fish..
What is the size in KDa of mouse IgG on western blot?
Running it on the SDS-PAGE and then immunoblotting (Western blotting) for it, you would get 2 bands: ~ 25 kDa band corresponding to the IgG light chain, and ~ 55 kDa band corresponding to the IgG heavy chain. If you don't use SDS-PAGE but rather a very weak detergent, you would most likely observe a band of 160 kDa, the size of the undentaured protein.
What is sds-page used for?
SDS - PAGE is apparently used to seperate proteins. The proteins are by nature different sizes. SDS works as a stabilizer by separating proteins according to similar forms.
What is the molecular weight of cellulase?
it is depend on the purity of pectinase enzyme. to know exact value of mw you have to run SDS page for individual sample.
What is Laemmli gels?
It is the gel of choice for SDS PAGE
What type of gene is the p53 gene?
p53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53gene. p53 is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressorthat is involved in preventing cancer. As such, p53 has been described as "the guardian of the genome" because of its role in conserving stability by preventing genome mutation.The name p53 is in reference to its apparent molecular mass: It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. But, based on calculations from its amino acid residues, p53's mass is actually only 43.7 kDa. This difference is due to the high number of prolineresidues in the protein, which slows its migration on SDS-PAGE, thus making it appear heavier than it actually is. This effect is observed with p53 from a variety of species, including humans, rodents, frogs, and fish..
What is the size in KDa of mouse IgG on western blot?
Running it on the SDS-PAGE and then immunoblotting (Western blotting) for it, you would get 2 bands: ~ 25 kDa band corresponding to the IgG light chain, and ~ 55 kDa band corresponding to the IgG heavy chain. If you don't use SDS-PAGE but rather a very weak detergent, you would most likely observe a band of 160 kDa, the size of the undentaured protein.
What is sds-page used for?
SDS - PAGE is apparently used to seperate proteins. The proteins are by nature different sizes. SDS works as a stabilizer by separating proteins according to similar forms.
What is observed when run a sds-page using the pellet from serum collection?
SDS is a type of polyacrylamide gel in which bacteria can be grown. To see what can be observed, the collection and experiment should be done by the student.
Why is denaturing sds-page used for running sds-page electrophoresis of egg-white lysozyme and not non-denaturing page?
may be because of toomany disulfide linkages
What is the function of glycine in sds page?
glycine molecular weight high so mobility also high so using in SDS PAGE
What is the molecular weight of cellulase?
it is depend on the purity of pectinase enzyme. to know exact value of mw you have to run SDS page for individual sample.
What are the drawbacks of sds page?
The major drawback is that treatment with SDS denatures the protein, meaning you are not looking at it in its natural state.
What is Laemmli gels?
It is the gel of choice for SDS PAGE
Who discovered SDS PAGE electrophoresis?
Laemmli U. K.
What test has replaced the radial immunodiffusion test?
SDS-PAGE method
What is role of glycine in SDS-PAGE?
Glycine increases the mobility of the gel.
