Zoology or Animal Biology
HIV and AIDS
Mice and Rats

What is the size in KDa of mouse IgG on western blot?

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Answer

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Wiki User
03/09/2009

Running it on the SDS-PAGE and then immunoblotting (Western blotting) for it, you would get 2 bands: ~ 25 kDa band corresponding to the IgG light chain, and ~ 55 kDa band corresponding to the IgG heavy chain. If you don't use SDS-PAGE but rather a very weak detergent, you would most likely observe a band of 160 kDa, the size of the undentaured protein.