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Q: What is the size in KDa of rabbit IgG on western blot?
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What is the size in KDa of mouse IgG on western blot?

Running it on the SDS-PAGE and then immunoblotting (Western blotting) for it, you would get 2 bands: ~ 25 kDa band corresponding to the IgG light chain, and ~ 55 kDa band corresponding to the IgG heavy chain. If you don't use SDS-PAGE but rather a very weak detergent, you would most likely observe a band of 160 kDa, the size of the undentaured protein.


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this kind of question how i know to answering but i have a opinion. firstly just back home and sleep