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What happens if grams iodine is not applied after staining with crystal violet?
Wiki User
∙ 7y ago
If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.
Wiki User
∙ 15y ago
Wiki User
∙ 15y agoI believe that Iodine helps the cell take up the Crystal Violet. If Iodine is not used and you did everything else the right way that you would get a false gram negative. The Violet would not get taken up like it should and the Safranin would end up staining a Gram positive cell red.
Wiki User
∙ 7y agoIf iodine is not added, the crystal violet will not stay (at least most of it will not) in the cell. Not using that step will invalidate the procedure. The reading may not be correct. The iodine acts as a mordant by binding to the crystal violet so that together, they will be too large to be removed.
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Why is Iodine used in Gram staining?
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
What are the reagents and steps min a Gram stain?
The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
What is the function of iodine solution in the gram stain?
It allows the primary stain crystal violet to remain in the cell instead of being washed out. Due to the larger size of the crystal violet molecule, when the ethanol is applied (the decolorizer) the stain will not be washed out of the Gram (+) positive cells.
Why does lugol's iodine stain a cell?
iodine indicates polysaccharides, therefore plant cells can be stained with iodine, staining the chloroplasts- composed of starch(a polysaccharide), and the cell wall- composed of cellulose ( a polysaccharide)
Why is Iodine used in Gram staining?
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
What is crystal violet iodine complex?
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
Function of Gram's iodine?
Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.
What happens when you add iodine crystal and chloroform?
iodine is soluble in CHCl3. Liquid become purple in color.
What are the reagents and steps min a Gram stain?
The steps in Gram staining are:1. crystal violet added to the smear2. iodine, the mordant (this fixes the violet)3. a decolorizer made of acetone and alcohol4. safranin, the counterstainIf the cell is Gram +, the decolorizer can not remove the violet. If it is Gram -, the decolorizer can remove the violet and the cell can be then colored with the dye, safranin.Bacteria are grouped in 4 groups by Gram stain:Gram-positive, the cell wall retains crystal Violet.Gram-negative, the cell wall does not retain crystal Violet.Graham not reactive, no staining whatsoever.Graham variable, uneven staining.
What function of lugol's iodine in gram staining?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
What can be used for a simple stain procedure?
Perhaps Gram Staining? Steps are as follows: 1. Crystal Violet, 2. Iodine, 3. Decolorizer, 4. Safrinin
Why iodine oinment called non-staining?
Usually iodine compounds leave a yellow-brown stain on whatever they touch. Non staining will reduce that chance.
What is the function of iodine solution in the gram stain?
It allows the primary stain crystal violet to remain in the cell instead of being washed out. Due to the larger size of the crystal violet molecule, when the ethanol is applied (the decolorizer) the stain will not be washed out of the Gram (+) positive cells.
What happens when iodine crystals are placed in water?
They will dissolve until the water is saturated then the remaining crystal will stay intact.
What is the purpose of mordant in a differential staining procedure?
The resultant crystal violet- iodine (CV-1) complex serves to intensify the-color of the stain. At this point, all cells will appear purple black.
Why is iodine important to your world?
iodine is used for disinfecting water while camping and staining specimens for microscope use
