0
Want this question answered?
Be notified when an answer is posted
Add your answer:
Earn +20 pts
What are the steps of HIV replication in the correct order?
reverse transcription -> attachment to CD4 cells -> integration
Why does DNA replication have to be in an anti-parallel direction?
DNA polymerase III can only work in the 5' to 3' direction.
What is the role of DNA ligase in the enlogation of the lagging strand during DNA replication?
it is a linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of one DNA fragment to the 5' end of another DNA fragment.
How many strands of DNA are used as templates during replication?
5'-3' : One strand
When DNA replicates what are the new DNA made of?
its made of DNA. Replication just doubles the DNA, just like when bacteria or humans replicate.. the new human is .. well made of human.... It is opposite and anti-parallel.... 5-AATGTC-3 Original strand 3-TTACAG-5 new strand Each new molecule will have 1 original strand, and 1 daughter/newly synthesized strand. DNA replication results in DNA DNA transcription results in a strand of RNA
Flow chart of 8 steps of DNA replication?
The eight steps of DNA replication are: 1. DNA strands separate, 2. formation of replication fork, 3. RNA primase binds, 4. bases pair up, 5. elongation, 6. RNA primers removed, 7.termination, 8. repair. this can occur in any cell.
What are the steps of HIV replication in the correct order?
reverse transcription -> attachment to CD4 cells -> integration
Why does DNA replication have to be in an anti-parallel direction?
DNA polymerase III can only work in the 5' to 3' direction.
What is the job of the DNA polymerases?
DNA polymerase adds bases to the 3' end during replication. It matches the c with G and A with U during replication. Never add to the 5' end!
The direction of DNA replication on the leading strand occurs in the?
The enzyme DNA polymerase synthesises strands in the 5 prime to 3 prime direction, and as DNA is antiparallel the replication of the leading strand occurs from the 3 prime end of the template to the 5 prime end of the template.
What is the role of DNA ligase in the enlogation of the lagging strand during DNA replication?
it is a linking enzyme essential for DNA replication; catalyzes the covalent bonding of the 3' end of one DNA fragment to the 5' end of another DNA fragment.
How many strands of DNA are used as templates during replication?
5'-3' : One strand
What is the function of DNA ligase in DNA replication?
it ties the chunks of dna together
What are the steps of DNA replication?
1)The first major step for the DNA Replication to take place is the breaking of hydrogen bonds between bases of the two antiparallel strands. The unwounding of the two strands is the starting point. The splitting happens in places of the chains which are rich in A-T. That is because there are only two bonds between Adenine and Thymine (there are three hydrogen bonds between Cytosine and Guanine). Helicase is the enzyme that splits the two strands. The initiation point where the splitting starts is called "origin of replication".The structure that is created is known as "Replication Fork".2) One of the most important steps of DNA Replication is the binding of RNA Primase in the the initiation point of the 3'-5' parent chain. RNA Primase can attract RNA nucleotides which bind to the DNA nucleotides of the 3'-5' strand due to the hydrogen bonds between the bases. RNA nucleotides are the primers (starters) for the binding of DNA nucleotides.3) The elongation process is different for the 5'-3' and 3'-5' template.a)5'-3' Template: The 3'-5' proceeding daughter strand -that uses a 5'-3' template- is called leading strandbecause DNA Polymerase ä can "read" the template and continuously adds nucleotides (complementary to the nucleotides of the template, for example Adenine opposite to Thymine etc).b)3'-5'Template: The 3'-5' template cannot be "read" by DNA Polymerase ä. The replication of this template is complicated and the new strand is called lagging strand. In the lagging strand the RNA Primase adds more RNA Primers. DNA polymerase å reads the template and lengthens the bursts. The gap between two RNA primers is called "Okazaki Fragments".The RNA Primers are necessary for DNA Polymerase å to bind Nucleotides to the 3' end of them. The daughter strand is elongated with the binding of more DNA nucleotides.4) In the lagging strand the DNA Pol I-exonuclease- reads the fragments and removes the RNA Primers. The gaps are closed with the action of DNA Polymerase (adds complementary nucleotides to the gaps) and DNA Ligase (adds phosphate in the remaining gaps of the phosphate - sugar backbone).Each new double helix is consisted of one old and one new chain. This is what we call semiconservative replication.5) The last step of DNA Replication is the Termination. This process happens when the DNA Polymerase reaches to an end of the strands. We can easily understand that in the last section of the lagging strand, when the RNA primer is removed, it is not possible for the DNA Polymerase to seal the gap (because there is no primer). So, the end of the parental strand where the last primer binds isn't replicated. These ends of linear (chromosomal) DNA consists of noncoding DNA that contains repeat sequences and are called telomeres. As a result, a part of the telomere is removed in every cycle of DNA Replication.6) The DNA Replication is not completed before a mechanism of repair fixes possible errors caused during the replication. Enzymes like nucleases remove the wrong nucleotides and the DNA Polymerase fills the gaps.Similar processes also happen during the steps of DNA Replication of prokaryotes though there are some differences.
When DNA is copied the molecule splits open along the?
When DNA is copied, the molecule splits open along the double helix. This occurs during the process of DNA replication.
DNA replication takes place when?
DNA replication takes place when preceding interphase.
What are the steps in DNA replication?
Primer made from primase attracts transcription factors, which call DNA-dependent DNA polymerase.DNA-dependent DNA polymerase binds while helicase unwinds the helix.Stable single-stranded DNA prevent the helix from re-annealing.DNA polymerase adds nucleotides in a 5' to 3' direction.RNAase replaces the RNA primerAnother DNA polymerase replaces nucleotides.Ligase joins them together.
