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cutting of DNA into fragments simply means application of suitable restriction enzyme to it.now a days two types of restriction enzymes are available,1)exonucleases,which cut at end portion of DNA and 2)endonucleases ,which cut at specific inner site.
Through the process of gel electrophoresis.
DNA can be fragmented using restriction endonucleases or restriction enzymes. Restriction enzymes identify specific sequences within the DNA and cause cleavage generating fragments. When this digested DNA is allowed to run in gel electrophoresis fragments get separated according to their mass. When visualized under UV transilluminator, fragmented DNA can be observed as fluorescing bands.
Electrophoresis. Restriction enzymes are used to cut DNA into fragments. Solutions containing these fragments are placed on the surface of a gel to which an electric current is applied. The electric current causes the DNA fragments to move through the gel. Because smaller fragments move more quickly than larger ones, this process separates the fragments according to size.
DNA is of a negative charge. So when gel electrophoresis is used on it the DNA fragments are attracted to the positive end of the electrophoresis. The fragments of different lengths travel down the gel towards this end. The longer length fragments travel less and so are farther from the positive end. By looking at these DNA fragments, which are created by cutting DNA with restriction enzymes one can compare and contrast DNA. Thus DNA fingerprinting can take place based on the different restriction sites in DNA (cut by the enzymes) forming different length segments of DNA.
cutting of DNA into fragments simply means application of suitable restriction enzyme to it.now a days two types of restriction enzymes are available,1)exonucleases,which cut at end portion of DNA and 2)endonucleases ,which cut at specific inner site.
two identical DNA fragments will have identical restriction fragments. Also, genetically identical twins will have identical restriction fragments
Through the process of gel electrophoresis.
The chemicals used to cut DNA fragments are restriction endonucleases, eg- Eco R I, Eco R II,etc
restriction enzymes or endonuclease enzymes
If the plasmid has 3 recognition sequences for a given restriction endonuclease, then 4 linear DNA fragments are obtained because, if the DNA is linear then the number of fragments obtained is (N+1) whereas if the DNA is circular then the number of fragments obtained will be N for N recognition sequences for the given restriction endonuclease in a plasmid.
A restriction enzyme (or restriction endonuclease) is an enzyme that cuts double-stranded or single stranded DNA at specific recognition nucleotide sequences called restriction sites.
They are used to show the lengths of DNA fragments between restriction sites in a strand of DNA.
DNA can be fragmented using restriction endonucleases or restriction enzymes. Restriction enzymes identify specific sequences within the DNA and cause cleavage generating fragments. When this digested DNA is allowed to run in gel electrophoresis fragments get separated according to their mass. When visualized under UV transilluminator, fragmented DNA can be observed as fluorescing bands.
Electrophoresis. Restriction enzymes are used to cut DNA into fragments. Solutions containing these fragments are placed on the surface of a gel to which an electric current is applied. The electric current causes the DNA fragments to move through the gel. Because smaller fragments move more quickly than larger ones, this process separates the fragments according to size.
DNA is of a negative charge. So when gel electrophoresis is used on it the DNA fragments are attracted to the positive end of the electrophoresis. The fragments of different lengths travel down the gel towards this end. The longer length fragments travel less and so are farther from the positive end. By looking at these DNA fragments, which are created by cutting DNA with restriction enzymes one can compare and contrast DNA. Thus DNA fingerprinting can take place based on the different restriction sites in DNA (cut by the enzymes) forming different length segments of DNA.
For DNA gel electrophoresis, yes. Once the DNA is cut up into different-sized fragments, they can be electrophoresed to separate bands.