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What do you think an enzyme like DNA polymerase would do?
VictoriaaaBeee
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Compare the polymerase chain reaction and DNA replication?
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
What affect would temperature and pH have on the Catalans enzyme?
Just like always, deviating from the desired normal functioning for the enzyme, whether it be in temperature or pH, would result in the enzyme denaturing and therefore being unable to for enzyme substrate complexes, therefore reducing the overall reaction rate.
How cofactor work?
Usually there are three kinds of proteins that could bind to an enzyme to enhance its catalytic activity. The prosthetic groups are one that are firmly attched to the enzymes. The best example of a prosthetic group is heme that is bound to hemoglobin. The next is co factor, that could dissociate itself from the enzyme, like NADH and NADPH. Cofactor, finally, are metal ions, usually cations, that bind to the metal ions and enhance the activity. they perform certain functions; as to hold the substrates in close proximity. The best example is DNA and RNA polymerase, where the upcoming nucleotide is held in the catalytic cleft of polymerase by Magnesium ions.
How is a catalyst like platinum different from an enzyme?
Catalysts are compounds that change the speed of chemical reactions. An enzyme is a protein and also a catalyst. So an enzyme can be a catalyst, but a catalyst can't be an enzyme.
Organelle responsible for the transportation of information?
The transportation of information in a cell occurs in two steps. Firstly DNA (the information store) is transcribed into mRNA this is done by enzymes like RNA polymerase. This mRNA is then tranlated into proteins by the RIBOSOME (this is the organelle i think your asking about)
Compare the polymerase chain reaction and DNA replication?
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
What enzyme catalyzes DNA replication?
one of them is heliocase. it 'unzips' the DNA strand. You can always remember this because it's in a popular joke: Q. Why is the enzyme heliocase a lot like a teenage boy? A. They both want to unzip your jeans (genes) !!!!!
Which enzyme is used specifically for rna transcription from dna in nucleus?
RNA polymerase.... What a wonderful, uplifting speech she gave at the convocation memorial service at Va Taech the day after the shootings there. She raised the spirits of both the students and the staff along with those of the world. She gave hope to the hopeless of the world and raised every single person involved with Va Tech to the highest of all highs. Va Tech is indeed lucky to have her as one of their professors. I hope and pray that they all recover from this horrible tragedy and with staff like her, the students will prevail! Go Hokies! Go Hokies!
Differences between DNA polymerase and RNA polymerase?
A polymerase is an enzyme that catalyzes the conversion of free nucleotides into a single strand. DNA polymerase differs from RNA polymerase in two major respects: * Like all enzymes, DNA polymerase is substrate-specific. DNA polymerase cannot extend a single strand of DNA; it needs at least a short segment of double-stranded DNA at the outset. * As its name implies, DNA polymerase incorporates deoxyribonucleotides into the new strand. RNA polymerase incorporates ribonucleotides. These differences mean that DNA polymerase is active when new DNA strands are formed, as in DNA replication, and RNA polymerase is active when new RNA is formed, as in transcription. Before DNA replication can begin, the two strands must uncoil, so that each can form a template for free nucleotides to attach to. But DNA polymerase cannot get started with a single strand! In vivo(in the cell) RNA polymerase, which is active in the presence of single-stranded DNA, catalyzes the incorporation of a handful of nucleotides into a new strand. The short length of double-stranded nucleic acid that is produced enables DNA polymerase to swing into action. This still leaves a potential difficulty: the nucleotides incorporated in the presence of RNA polymerase are the wrong sort (ribonucleotides). They are subsequently replaced by DNA polymerase. In vitro (during PCR, the polymerase chain reaction) a primer, specially synthesized in a laboratory, attaches to a specific segment of single-stranded DNA, and the DNA polymerase takes over from there. The primer consists of a short length of single-stranded DNA that uniquely complements a specific DNA segment that is targeted for amplification, for example for forensic analysis.In practice, there are several different DNA polymerases and RNA polymerases in an organism.
What enzyme can remove or insert super coil twists into circular DNA?
The enzyme topoisomerase is used in inserting or loosing supercoiling in DNA during replication. It is of different type like gyrase, helicase etc. and are found in prokaryotes and eukaryotes respectively
What affect would temperature and pH have on the Catalans enzyme?
Just like always, deviating from the desired normal functioning for the enzyme, whether it be in temperature or pH, would result in the enzyme denaturing and therefore being unable to for enzyme substrate complexes, therefore reducing the overall reaction rate.
What nucleic acids are involved in translation?
There are a variety of enzymes used in replication. Helicase is used to open the hydrogen bonds that connect the two strands. However, this causes a tension to form in the strands (like a wind up toy) so some of it needs to be released. This is done by topoisomerase, which cuts the strands, lets them spin out some of the tension and attaches the DNA back together again. Moving behind helicase, is an enzyme called SSBP. This basically binds to the DNA sequence to prevent it from reattaching to itself after helicase unzips it; DNA would otherwise just bond back with the other strand. Then an RNA Polymerase called primase comes and attaches a primer to the DNA strands. This is needed because the next enzyme, DNA polymerase will not from scratch and needs a base to work from: the primer serves this role. Starting on the primer, DNA Polymerase III synthesizes the new strand, but the primers are still left on the strands. These will be removed by DNA Polymerase I which also adds new nucleotides to the hole left by the primer. Finally, an enzyme called ligase fills the one nucleotide gap left between the primer and the newly synthesized DNA with a sugar phosphate backbone (not another nucleotide)
What would happento the cellular respiration process if the enzyme for one step of the process was missing?
If an enzyme in a sequence of enzyme-controlled reactions is missing or defective then the process will stop at that point. So respiration could proceed until it reached the reaction which needed the missing or defective enzyme at which point it would stop.
Why can't DNA polymerase synthesize a new strand of DNA without the help of a RNA primer?
Because DNA Polymerase requires the OH on the 3' as an active site. It uses the OH on the 3' end of a nucleotide to attach a phosphate from the 5' end of the next nucleotide. It only works in this direction, and that is why DNA polymerase works 5' to 3'.
What are the enzymes involved in replication?
There are a variety of enzymes used in replication. Helicase is used to open the hydrogen bonds that connect the two strands. However, this causes a tension to form in the strands (like a wind up toy) so some of it needs to be released. This is done by topoisomerase, which cuts the strands, lets them spin out some of the tension and attaches the DNA back together again. Moving behind helicase, is an enzyme called SSBP. This basically binds to the DNA sequence to prevent it from reattaching to itself after helicase unzips it; DNA would otherwise just bond back with the other strand. Then an RNA Polymerase called primase comes and attaches a primer to the DNA strands. This is needed because the next enzyme, DNA polymerase will not from scratch and needs a base to work from: the primer serves this role. Starting on the primer, DNA Polymerase III synthesizes the new strand, but the primers are still left on the strands. These will be removed by DNA Polymerase I which also adds new nucleotides to the hole left by the primer. Finally, an enzyme called ligase fills the one nucleotide gap left between the primer and the newly synthesized DNA with a sugar phosphate backbone (not another nucleotide)
Can polymerase catch and correct every reptication error?
Yes. Initially, DNA replication makes 1 mistake in a 100,000. Like spell check, DNA polymerase comes in and removes errors in base pairs and correct them by adding the right ones. After DNA polymerase checks the new strand for errors, the end result is 1 mistake in a billion. If this didn't occur, mutations would surely take place in out body.
How cofactor work?
Usually there are three kinds of proteins that could bind to an enzyme to enhance its catalytic activity. The prosthetic groups are one that are firmly attched to the enzymes. The best example of a prosthetic group is heme that is bound to hemoglobin. The next is co factor, that could dissociate itself from the enzyme, like NADH and NADPH. Cofactor, finally, are metal ions, usually cations, that bind to the metal ions and enhance the activity. they perform certain functions; as to hold the substrates in close proximity. The best example is DNA and RNA polymerase, where the upcoming nucleotide is held in the catalytic cleft of polymerase by Magnesium ions.
