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If you are referring to the "Plant Pigments and Photosynthesis" Lab Then, the DPIP is used to substitute NADP+. In photosynthesis, electrons are normally transferred to NADP+. However, DPIP will take its place in this experiment. DPIP is normally blue. When it is reduced, or gains electrons, it will turn colorless. DPIP is used to show that photosynthesis is taking place.
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∙ 15y ago
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∙ 11y ago2,6-dichlorophenolindophenol
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What is the function of DPIP in this experiment?
Is this an AP lab?The DPIP replaces NADP+.In photosynthesis, NADP is reduced to NADPH, but NADP is colorless.DPIP replaces the NADP. When DPIP is reduced, it changes from blue to clear. DPIP is used to show photosynthesis taking place.
What is the effect of darkness on the reduction of DPIP Explain?
Darkness means no reduction. NADP is in light reactions - DPIP is electron acceptor susbstitute for NADP... if NADP does not react in darkness (plants dont react at night and do not undergo photosynthesis) then neither should DPIP
Are boiled chloroplasts able to reduce DPIP?
Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.
What would perform the same function as DPIP in the living plant?
NADP + would perform the same function. During photosynthesis electrons are transferred there.
What reasons can you give for the difference in the percentage of transmittance between the live chlorplats that were incubated in the light and those that were kept in the dark?
The percent transmittance grew to steadily higher numbers as the experiment progressed because the light reaction was able to occur. However, the dark cuvettes had stable levels of transmittance because light is necessary to excite electrons, which, in turn, reduces the DPIP.
Electron capture by DPIP?
DPIP substitutes for electron acceptor
What is the function of DPIP in this experiment?
Is this an AP lab?The DPIP replaces NADP+.In photosynthesis, NADP is reduced to NADPH, but NADP is colorless.DPIP replaces the NADP. When DPIP is reduced, it changes from blue to clear. DPIP is used to show photosynthesis taking place.
Why wasn't dpip added to cuvette 1?
Because it was the control. Note how all other cuvettes had DPIP. We did not really know what dpip's effect on the % light transmittance was, by adding a ontrol, we could not compare and contrast.
What role DPIP plays photosynthesis?
In lab 4, DPIP is an electron acceptor that takes the place of NADP+. When DPIP is reduced, it changes from blue to colorless so you can see that the Calvin cycle is running.
What is the effect of darkness on the reduction of DPIP Explain?
Darkness means no reduction. NADP is in light reactions - DPIP is electron acceptor susbstitute for NADP... if NADP does not react in darkness (plants dont react at night and do not undergo photosynthesis) then neither should DPIP
What is the source of electrons that will reduce DPIP?
the electrons came from splitting water molecules and were passed to the chlorophyll molecules, eventually being transferred to the DPIP
What is the effect of boiling the chloroplast on the subsequent?
Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.What_is_the_effect_of_boiling_chloroplasts_on_the_subsequent_reduction_of_DPIP
Are boiled chloroplasts able to reduce DPIP?
Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.
What molecule found in chloroplast does 2.6-dichlorophenol-indophenol replace?
DPIP substitutes for the NADP+ molecules.
What would perform the same function as DPIP in the living plant?
NADP + would perform the same function. During photosynthesis electrons are transferred there.
What reasons can you give for the difference in the percentage of transmittance between the live chlorplats that were incubated in the light and those that were kept in the dark?
The percent transmittance grew to steadily higher numbers as the experiment progressed because the light reaction was able to occur. However, the dark cuvettes had stable levels of transmittance because light is necessary to excite electrons, which, in turn, reduces the DPIP.
What is the purpose of AP lab 4?
Exercise 4A: To separate and identify the various pigments (which absorb sunlight) in spinach leaves. Exercise 4B: To study the light-dependent reactions. We will be measuring the rate of DPIP reduced and the percent light transmittance.
