the electrons came from splitting water molecules and were passed to the chlorophyll molecules, eventually being transferred to the DPIP
DPIP (2,6-dichlorophenolindophenol) is used as an artificial electron acceptor in laboratory experiments to measure the rate of photosynthesis. It acts as an electron carrier, accepting electrons from photosystem I in the light reactions of photosynthesis. By monitoring the reduction of DPIP from blue to colorless, researchers can quantify the rate of electron transfer and ultimately the rate of photosynthesis.
Hydrogen ions and a pair of electrons are added to NAD+ to reduce it to NADH. This reduction reaction is important in cellular respiration for the generation of ATP.
The source of hydrogen (H) for the Calvin cycle in photosynthesis comes from water molecules. During the light-dependent reactions, water is split by the enzyme complex in the thylakoid membrane, releasing oxygen, protons (H+), and electrons. The electrons are then used to reduce NADP+ to NADPH, providing the reducing power needed for the Calvin cycle to convert carbon dioxide into carbohydrates.
The source of oxygen in photosynthesis in plants is water. During the light-dependent reactions of photosynthesis, water molecules are split into oxygen, protons, and electrons. The oxygen is released into the atmosphere as a byproduct of this process.
The energy source of an electron microscope is electricity. It uses high-voltage electrical currents to generate and control a beam of electrons that are focused and scanned across a sample to create magnified images.
If you are referring to the "Plant Pigments and Photosynthesis" Lab Then, the DPIP is used to substitute NADP+. In photosynthesis, electrons are normally transferred to NADP+. However, DPIP will take its place in this experiment. DPIP is normally blue. When it is reduced, or gains electrons, it will turn colorless. DPIP is used to show that photosynthesis is taking place.
Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.
Darkness means no reduction. NADP is in light reactions - DPIP is electron acceptor susbstitute for NADP... if NADP does not react in darkness (plants dont react at night and do not undergo photosynthesis) then neither should DPIP
Adding more DPIP to each tube would likely result in a faster color change, as more DPIP molecules would be available to accept electrons from the reduced chloroplasts. This would lead to a quicker reduction of DPIP and a faster transition from blue to colorless, indicating a higher rate of photosynthesis.
In the succinate-fumarate step, electrons are transferred from succinate to FAD to form FADH2, which eventually reduces quinone to quinol. This reduction reaction leads to a color change in DPIP, indicating the transfer of electrons from succinate to the electron transport chain.
NADPH
Is this an AP lab?The DPIP replaces NADP+.In photosynthesis, NADP is reduced to NADPH, but NADP is colorless.DPIP replaces the NADP. When DPIP is reduced, it changes from blue to clear. DPIP is used to show photosynthesis taking place.
DPIP (2,6-dichlorophenolindophenol) is used as an artificial electron acceptor in laboratory experiments to measure the rate of photosynthesis. It acts as an electron carrier, accepting electrons from photosystem I in the light reactions of photosynthesis. By monitoring the reduction of DPIP from blue to colorless, researchers can quantify the rate of electron transfer and ultimately the rate of photosynthesis.
NADPH
Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.What_is_the_effect_of_boiling_chloroplasts_on_the_subsequent_reduction_of_DPIP
Adding more DPIP to each experimental tube would likely result in a faster rate of color change or a more pronounced color change in the experiment. DPIP is a redox indicator that changes color as it accepts electrons during the photosynthetic process. Increasing the amount of DPIP can make the color change more noticeable due to a higher concentration of the indicator molecule being reduced.
Because it was the control. Note how all other cuvettes had DPIP. We did not really know what dpip's effect on the % light transmittance was, by adding a ontrol, we could not compare and contrast.