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What does competent refers to an competent cells used in transformation experiment?
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Why gene gun use in rice transformation?
It's a tool to insert foregin gene into target cells and can be used for any suitable crop or cells for transformation purpose. Now a days, agrobacterium mediated transformation techniques are becoming popular.
The host organism into which a cloning vector is placed is called?
Competent cells if i not mistaken
What is the process by which bacteria cells pick up and incorporate DNA from dead bacteria cells?
transformation
What is the definition of competent cells and how it can help to make a transformation more efficient during the cloning of desired gene?
Generally most of the bacteria have the ability to take up DNA which is present freely in the environment. This is called Competence. It is genetically acquired. But some cells do not exhibit natural competence and they are treated with a suitable chemical like CaCl2 to make their cell wall relatively permeable to DNA. We can make any cell competent in vitro and introduce our desired gene which is taken up by the competent cell. To test the efficiency of our treatment, we may introduce a marker gene like gene for antibiotic resistance such as Tetracyclin onto the desired gene and grow the treated cells on Tetracyclin medium so that only the cells that have taken up the DNA survive.
What did Fred Griffith discover when he injected mice with a mixture of dead pathogenic S cells and living harmless r cells of Streptococcus pneumonia?
Griffith had discovered what is now called Transformation, transformation is a change in genotype caused when cells take up foreign genetic material.
What is the definition of competent cells?
cells which readily accept a foreign DNA through a process called transformation
How molecular mechanism of bacterial transformation is carried out?
Basically, transformation refers to the uptake and incorporation of naked genetic material by a competent cell. Most bacterial cells are not naturally competent(able to take up naked genetic material). The kinds that are use a type 4 pilli to get the genetic material into the cell. This process is very rare in nature, since it requires naked genetic material to be present, from a lysed cell, as well as a competent cell to take up the genetic material. When the DNA/RNA is taken up, it is often degraded by restriction enzymes. If it is not degraded it can exist as extra chromosomal DNA, much like a plasmid, or it can be incorporated into the cells chromosome. Scientists use heat, pH, or osmotic shock in lab to make cells artificially competent.
Can cells be transformed when they are in a competent state?
They can only be transformed when they are in a competent state.
What are some reasons why transformation may not be successful?
One factor could have been that the cells were not completely competent. Also, some colonies take longer to grow so a longer incubation time may have helped.
What is The transfer of DNA into cells?
Transformation
Transfer of DNA into cells is called what?
Transcription
What do control setup and experimental setup have in common?
Everything except what you're measuring. EXAMPLE: In a biological experiment using specially treated cells the experiment is run with the cells; this is the experiment. Then the experiment is run again with the same cells WITHOUT the special treatment; this is the control.
Why gene gun use in rice transformation?
It's a tool to insert foregin gene into target cells and can be used for any suitable crop or cells for transformation purpose. Now a days, agrobacterium mediated transformation techniques are becoming popular.
The host organism into which a cloning vector is placed is called?
Competent cells if i not mistaken
What is the process by which bacteria cells pick up and incorporate DNA from dead bacteria cells?
transformation
What is the definition of competent cells and how it can help to make a transformation more efficient during the cloning of desired gene?
Generally most of the bacteria have the ability to take up DNA which is present freely in the environment. This is called Competence. It is genetically acquired. But some cells do not exhibit natural competence and they are treated with a suitable chemical like CaCl2 to make their cell wall relatively permeable to DNA. We can make any cell competent in vitro and introduce our desired gene which is taken up by the competent cell. To test the efficiency of our treatment, we may introduce a marker gene like gene for antibiotic resistance such as Tetracyclin onto the desired gene and grow the treated cells on Tetracyclin medium so that only the cells that have taken up the DNA survive.
Why do you keep competent cells in ice?
to avoid denaturation of the genetic material in this case DNA
