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What does it mean that Wash organic layer with 1.0 ml of 1M aqueous sodium bicarbonate?
Wiki User
∙ 14y ago
1m is the molarty of the solution meaning the concentration is 1 mol of baking soda (sodium bicarbonate) to every liter of water. 1 mol of sodium bicarbonate is 84g. so for 1ml of water with a molarity of 1 divide 84g by 1000 which is 84mg and add that to 1ml of water. as far as wahing the organic layer i think it means washing any water from the solution but i may be wrong idk.
Wiki User
∙ 14y ago
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When you are extracting an aqueous solution with an organic solvent you are uncertain of which layer in the separatory funnel is aqueoushow would you quickly sort out the issue?
Add a drop of water and see if it mixes with the top layer or if it remains as a droplet within the top layer. If it mixes homogeneously, then the top is aqueous. If it forms a droplet, then the top is organic.
What is the purpose of water washing the organic layer?
The purpose of water washing the organic layer is for aqueous extraction to remove impurities and or reagents that are water soluble. For instance, in reactions that utilize a small tertiary amine base or inorganic base.
What is the advantage of having the organic solvent be denser then water when extracting an organic compound?
The difference in density allows you to separate out the components. DCM is much more dense than water, so any compounds that are dissolved in DCM will stay below the aqueous layer. This is extremely useful in acid/base reactions.
What is theFunction of chloroform in ctab method for DNA extraction?
Chloroform denaturizes the proteins and facilitates the separation of the aqueous and organic phase. If needed the extraction with chloroform is performed two or three times to completely remove the impurities from aqueous layer..
Explain briefly how you would separate chloroform or dichloromethane extract from an aqueous solution using a separating funnel?
Both chloroform and dichloroform are denser than water. You will see two layer in separating funnel. The lower layer will be chloroform and upper layer would be aqueous layer.
Why the aqueous layer is below the organic layer in the separating funnel?
The density of the aqueous layer is higher.
What is the purpose of the saturated NaCl solution for washing an organic layer why not use deionized water?
The bulk of the water can often be removed by shaking or "washing" the organic layer with saturated aqueous sodium chloride. The salt water works to pull the water from the organic layer to the water layer. This is because the concentrated salt solution wants to become more dilute and because salts have a stronger attraction to water than to organic solvents. Note: sometimes a saturated aqueous solution of sodium chloride is called brine.
The role of sodium chloride in extraction method?
Brine (saturated sodium chloride solution) is usually the last solution used in an aqueous wash to help remove trace amounts of water (and anything water soluble) from the organic layer. Many chemists skip this step however, since sodium sulfate or manganese sulfate is used to remove water from the organic layer anyway, after the organic layer is separated.
Why in cannizzaro reaction the aqueous layer become the bottom layer and the organic layer become the upper layer in the separating funnel?
Because the density of water more than the density of organic compounds
Is ethanol lipid soluble?
Yes. When mixed with water in a solution it forms an organic layer ontop of the aqueous (water) layer.
When you are extracting an aqueous solution with an organic solvent you are uncertain of which layer in the separatory funnel is aqueoushow would you quickly sort out the issue?
Add a drop of water and see if it mixes with the top layer or if it remains as a droplet within the top layer. If it mixes homogeneously, then the top is aqueous. If it forms a droplet, then the top is organic.
What is the purpose of water washing the organic layer?
The purpose of water washing the organic layer is for aqueous extraction to remove impurities and or reagents that are water soluble. For instance, in reactions that utilize a small tertiary amine base or inorganic base.
Why is anhydrous sodium sulfate used in organic chemistry labs?
Because of its affinity for water, anhydrous sodium sulfate is often used to dry hydrophobic solvents used in aqueous organic extractions. For example a water based sample is extracted for organic compounds using dichloromethane, or ethyl ether in a separatory funnel. The organic layer is passed through a buchner funnel holding anhydrous sodium sulfate, yielding dried solvent extract.
What is the advantage of having the organic solvent be denser then water when extracting an organic compound?
The difference in density allows you to separate out the components. DCM is much more dense than water, so any compounds that are dissolved in DCM will stay below the aqueous layer. This is extremely useful in acid/base reactions.
What is theFunction of chloroform in ctab method for DNA extraction?
Chloroform denaturizes the proteins and facilitates the separation of the aqueous and organic phase. If needed the extraction with chloroform is performed two or three times to completely remove the impurities from aqueous layer..
What are the benefits of including sodium bicarbonate as an ingredient in toothpaste?
Sodium bicarbonate, or baking soda, is used as a cleaning agent in toothpaste. It is especially effective for managing stains and odors, since it penetrates into the teeth for a deep cleanse. Compared to other cleansing agents, it is not very abrasive and therefore will cause little harm to the protective layer of the teeth known as the enamel.
What is an organic layer?
Organic layers are part of the soil.
