0
What do DNA bands represent in the agarose gel electrophoresis?
Wiki User
∙ 15y ago
Each band represents a piece of DNA. The extent to which they move through the gel has to do with the fragment's electrophoretic mobility. The lighter the molecule in general the faster it can move through the gel. Usually when performing a gel electrophoresis one would use markers. These markers would be of known molecular weight and would allow you to compare your DNA fragments and find approximate molecular weights.
Wiki User
∙ 15y ago
Wiki User
∙ 13y agoEach stained bands represents DNA fragments of particular size
Add your answer:
Earn +20 pts
What is the functions of UV transilluminator in genetic laboratory?
to vizualise DNA after Agarose gel electrophoresis
What is the function of agarose gel electrophoresis?
Agarose gel electrophoresis is for determining the size of a piece of RNA or DNA. It can be used to determine the culprit of a crime, relatives, even the cause of some diseases, like Mad Cow.
What is an agarose gel and what is it used for?
Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
Analysis of DNA fragments in gel electrophoresis involves what?
i)- the size of the DNA fragment, ii)- the density of the agarose gel, iii) - the intensity of the migratory electric field.
Process that restriction fragments of DNA are separated from each other by the use of electricity?
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
What is role of agarose of electrophoresis?
agarose helps in the separation of DNA bands by controlling the pore size of agarose gel
Is agarose gel electrophoresis suitable for only gram negative plasmid DNA?
Agarose gel electrophoresis is suitable for ALL DNA.
Function of agarose in agarose gel electrophoresis?
Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to make particles move. For example, DNA is negative, so it'll travel towards to positive electrode of the gel box. Agarose has small pores through which a DNA can travel. Bigger fragments of DNA travel shorter distances, because it takes longer for them to navigate through the pores of the agarose gel. Identically sized pieces of DNA will travel the same distance, which is why you get bands (DNA with loading dye) after you run a a gel.
Factors that affect the rate of DNA migration in Agarose gels electrophoresis.?
size of the DNA
What is the principle for agarose gel electrophoresis?
it is a technique that separated dna according to its size.
What is the functions of UV transilluminator in genetic laboratory?
to vizualise DNA after Agarose gel electrophoresis
what is the gel in Gel Electrophoresis?
For larger molecules like proteins we use polyacrylamide gel electrophoresis (PAGE). For smaller pieces like DNA we use agarose gel electrophoresis
What is the function of agarose gel electrophoresis?
Agarose gel electrophoresis is for determining the size of a piece of RNA or DNA. It can be used to determine the culprit of a crime, relatives, even the cause of some diseases, like Mad Cow.
What is the role of EtBr in electrophoresis?
Ethidium bromide interchalates with DNA. It doesn't affect electrophoresis, but it help visualise the DNA bands after electrophoresis. The EtBr that is bound to the DNA will fluoresce under ultraviolet light.
What is an agarose gel and what is it used for?
Agarose gel electrophoresis is the easiest and most common method used in biochemistry and molecular biology in separating DNA or RNA molecules according to their size.
Analysis of DNA fragments in gel electrophoresis involves what?
i)- the size of the DNA fragment, ii)- the density of the agarose gel, iii) - the intensity of the migratory electric field.
Process that restriction fragments of DNA are separated from each other by the use of electricity?
The process is referred to as gel electrophoresis. This is an analytical process where DNA fragments can be separated based on size within a gel under the influence of an electric field
