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The pH is varied to effect, by its affect, this test.
Different enzymes work best at different pH. This is refered to as the ideal pH for the enzyme. For example, the digestive enzyme trypsin works best at an acidic pH while alkaline phosphatase works best at a basic pH. Therefore, enzyme activity varies with pH and this variation depends on the enzyme being studied
temperature, pH, concentration of enzymes, and concentration of substrates.
Oxygen is the product of the catalase reaction causes bubbling. pH is varied when testing the effect of pH on enzyme activity.
temperature,pH and substrate concentration
pH
The pH is varied to effect, by its affect, this test.
Different enzymes work best at different pH. This is refered to as the ideal pH for the enzyme. For example, the digestive enzyme trypsin works best at an acidic pH while alkaline phosphatase works best at a basic pH. Therefore, enzyme activity varies with pH and this variation depends on the enzyme being studied
temperature and pH
The enzyme has an optimal point of pH at which the enzyme works best. For example a catalase enzyme works best in a pH of 7. When the pH changes it denatures the enzyme causing it to not be able to react with the substrate.
temperature, pH, concentration of enzymes, and concentration of substrates.
effect of ph and concentration of subtrate
The concentration of hydrogen ions in solution affects the enzyme activity. Each enzyme has maximal efficiency under an optimum pH. Since pH is one of the factors for the denaturation of proteins, if an enzyme is submitted to a pH level under which it is denatured there will be no enzymatic activity.
Ph level, enzyme concentration, substrate concentration etc
Denature enzyme activity
Oxygen is the product of the catalase reaction causes bubbling. pH is varied when testing the effect of pH on enzyme activity.
temperature,pH and substrate concentration