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What effect would doubling the length of the chromatography paper have on the Rf values?
Wiki User
∙ 14y ago
Not much, the separation in Paper Chromatography could be improved but also the diffusion (vagueness of the spots) and the run time.
In rising P-Chrom. the capillary forces may become too weak to go up further, and evaporation also can have negative effects. This can be omitted by hanging P-Chrom.
Wiki User
∙ 14y ago
Wiki User
∙ 14y agoSince the Rf value is a ratio, it would stay the same. The pigments might travel further, but so will the solvent.
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How does temperature affect chromatography?
The effect of temperature on retention characteristics in thin-layer chromatography (TLC) with low-volatility mobile phases (MP): It is shown that temperature variations in TLC in melts bring about variations in both the relative retention values and, in some cases, in the order of migration of the chromatographic zones across the layer.Hope that helped :)
What are the variables in a plan and design paper chromatography lab?
responding variable-distance of components( Rf values)
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See related link below for a better explanation than mine.. I am not to familiar with Rf values, but I have an idea. Rf values are the ratio between the distance traveled by the spot of the analyte to the distance traveled by the solvent used. This is used in paper chromatography and thin layer chromatography. Rf value will allow you to compare to your unknown substance that you are trying to analyze. I will put a link the both paper and TL chromatography.
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How does temperature affect chromatography?
The effect of temperature on retention characteristics in thin-layer chromatography (TLC) with low-volatility mobile phases (MP): It is shown that temperature variations in TLC in melts bring about variations in both the relative retention values and, in some cases, in the order of migration of the chromatographic zones across the layer.Hope that helped :)
What are the variables in a plan and design paper chromatography lab?
responding variable-distance of components( Rf values)
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What is the significance of Rf value in chromatography?
See related link below for a better explanation than mine.. I am not to familiar with Rf values, but I have an idea. Rf values are the ratio between the distance traveled by the spot of the analyte to the distance traveled by the solvent used. This is used in paper chromatography and thin layer chromatography. Rf value will allow you to compare to your unknown substance that you are trying to analyze. I will put a link the both paper and TL chromatography.
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