0
Would RF values of amino acid you use in paper chromatography be the same if you use another solvent system?
Wiki User
∙ 11y ago
Rf value is the ratio of distanced covered by solute by distanced covered by solvent.
Wiki User
∙ 11y ago
Add your answer:
Earn +20 pts
What is the significance of Rf value in chromatography?
See related link below for a better explanation than mine.. I am not to familiar with Rf values, but I have an idea. Rf values are the ratio between the distance traveled by the spot of the analyte to the distance traveled by the solvent used. This is used in paper chromatography and thin layer chromatography. Rf value will allow you to compare to your unknown substance that you are trying to analyze. I will put a link the both paper and TL chromatography.
What role does the chromatography plays in identification of the unknown?
Attaining individual Rf values for eachn ion in solvent under the same conditions allows us to compare numerical data, as opposed to just colours, in determining the composition of the unknown. -NL
How can proteins be analyzed by chromatography?
A solution of amino acids is spotted onto a piece of chromatography paper which is then placed into a container filled with a suitable solvent. A dye is used so that the position of the amino acids along the piece of paper can be seen. The distances travelled by the amino acids are measured to calculate their retention factors (Rf) values. These are then compared to known standards.
How different colors are discovers what colors are in each ink using chromatography?
The different substances present in the ink have different Rf values (Rf value is the ratio of the distance the dissolved material traveled to distance the solvent traveled) the different substances each have a unique or nearly unique Rf value. This occurs because each material's chemical structure has a different solubility in the solvent,used to develop the chromatography plate,. This means that some compounds easily dissolve and are carried by the solvent while others are more attracted to the chromatgraphy plate and don't budge as easily. Hope this helped. sincerely, a chem major
How would Rf values changes of Cations if the developing chamber left uncovered in paper Chromatography?
The solvent would keep on evaporating from the chromatogram and as a result it would keep on bringing the cations up, causing an increase in the Rf value.
What are the significance of Retention factor values?
Rf Values determine the solubility of a substance with respect to a certain solvent. It also determines the affinity of the solute to the solvent (greater Rf=greater affinity of solute to the solvent)
What is the significance of Rf value in chromatography?
See related link below for a better explanation than mine.. I am not to familiar with Rf values, but I have an idea. Rf values are the ratio between the distance traveled by the spot of the analyte to the distance traveled by the solvent used. This is used in paper chromatography and thin layer chromatography. Rf value will allow you to compare to your unknown substance that you are trying to analyze. I will put a link the both paper and TL chromatography.
How and why did the relative front values differ for each solvent system?
good
How would you expect Rf values to change if you went from hexane-ethyl acetate solvent system to ethyl acetate only?
the rf values would increase
Why are Rf values always less than 1?
Rf values are distance of substance from origin divided by distance of solvent front from origin. As the substance travels with the solvent, the solvent will always have a greater or equal value to the distance travelled by substance. This means the highest value of Rf is 1.
What role does the chromatography plays in identification of the unknown?
Attaining individual Rf values for eachn ion in solvent under the same conditions allows us to compare numerical data, as opposed to just colours, in determining the composition of the unknown. -NL
How can proteins be analyzed by chromatography?
A solution of amino acids is spotted onto a piece of chromatography paper which is then placed into a container filled with a suitable solvent. A dye is used so that the position of the amino acids along the piece of paper can be seen. The distances travelled by the amino acids are measured to calculate their retention factors (Rf) values. These are then compared to known standards.
How you might use Rf values to identify an unknown substance?
Although Rf values technically remain constant if the following are held constant: * solvent system * adsorbent * thickness of the adsorbent * amount of material spotted * temperature They are generally not used to identify an unknown substance specifically, since these factors are sometimes difficult to control. Most generally, during a chromatography experiment an unknown sample can be compared directly to a known sample simultaneously, i.e. they will have similar Rf values.
What are the significance of values?
Rf Values determine the solubility of a substance with respect to a certain solvent.
How different colors are discovers what colors are in each ink using chromatography?
The different substances present in the ink have different Rf values (Rf value is the ratio of the distance the dissolved material traveled to distance the solvent traveled) the different substances each have a unique or nearly unique Rf value. This occurs because each material's chemical structure has a different solubility in the solvent,used to develop the chromatography plate,. This means that some compounds easily dissolve and are carried by the solvent while others are more attracted to the chromatgraphy plate and don't budge as easily. Hope this helped. sincerely, a chem major
What do the Rf values indicate about the relative solubility of the pigment in the the solvent?
The Rf values do not indicate the solubility of a substance. The Rf value or retardation factor is the ratio of the distance traveled by the center of a pot to the distance traveled by the solvent front in chromatography.
How would Rf values changes of Cations if the developing chamber left uncovered in paper Chromatography?
The solvent would keep on evaporating from the chromatogram and as a result it would keep on bringing the cations up, causing an increase in the Rf value.
