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When being tested with biuret reagent, protein turns a blue to lilac color. However, starches when tested do change to a blackish blue color.
Proteins are made of peptide bonds which is the target of reaction for biuret test. It's not truly specific for proteins; biuret itself also gives a positive result (hence the name of the test). However, proteins are fairly common and biuret (and similar peptide-containing compounds) are relatively rare.
in a variegated leaf only the green areas of the leaf turns blue black in colour when tested for starch what is the best way to explan that
One possibility is a 10% solution of hydrochloric acid.
Benedicts solution changes through a range of colours (blue, green, orange, red) according to how much reducing sugar is present in the sample. This can be used to give a rough answer to the question "How much sugar is in the sample?", but is not accurate enough (because of the blending between one colour and the next), to be called a quantitative test.
Both cows milk and water-buffalo's milk produce a violet colour when tested with biuret solution because they both contain protein.
When being tested with biuret reagent, protein turns a blue to lilac color. However, starches when tested do change to a blackish blue color.
negative
By dissolving the pH solution a colour change will occur in the solution that should be tested.Compairing the colour of solution to the colours marked on of pH chart we can find out if the solution we tested is of alkaline nature or of acidic nature or neutral.Solutions of pH7are neutral solutions.Solutions below pH7 are acidic solutions and those above pH7 are alkaline solutions.
1) Add 2cm3 of solution to be tested to a test-tube. 2) Add an equal volume of 5% potassium hydroxide* solution and mix. 3) Add 2 drops of 1% copper sulphate solution and mix. A mauve or purple colour develops if protein is present. *Sodium hydroxide solution can be used instead of potassium hydroxide solution.
To test for protein. Biuret or solutions of sodium hydroxide and copper sulfate. Tested with a dropper a + result will be purple or something
Benedict's solution tests for aldehyde which is present in reducing sugars. If the solution remained blue than no reducing sugar's are present in corn oil.
Proteins are made of peptide bonds which is the target of reaction for biuret test. It's not truly specific for proteins; biuret itself also gives a positive result (hence the name of the test). However, proteins are fairly common and biuret (and similar peptide-containing compounds) are relatively rare.
How to test for protein - The Biuret test1. Add Sodium hydroxide to a 1% solution of albumen (the protein in egg white) or the ground food you want to be tested for protein2. Then add 1% copper sulphate solution3. If protein is present the solution will go purple
positive result for when buiret is added to protein is blue or violet/pale lilac colour. (not pink). positive result for when iodine is added to starch is blue or black colour (not pink).
It change from orange/brown colour to blue/black if starch is present
It turns that colour because it contains a small amount of starch.