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What form of technology uses electricity to sort DNA fragments based on their sizes?
Wiki User
∙ 12y ago
gel electrophorsis
Wiki User
∙ 12y ago
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Why are D1S80 fragments separated by gel electophoresis?
Fragments are separated by gel electrophoresis because of their differing sizes. DNA is negatively charged, so will migrate through the gel towards the positive electrode. The smaller fragments are able to move through the gel more quickly than the larger fragments - which means they separate based on their size.
Gel electrophoresis separates DNA fragments on the basis of differences in their?
Length. DNA has a natural negative charge - and so will move towards the positive electrode. Larger fragments move more slowly than shorter ones - so the sizes of fragments can be determined.
Properties of DNA fragments which allow them to be separated from each other during gel electrophoresis?
They are negatively charged and are of different sizes
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* Heterogeneous. (All different sizes). * Jagged fragments. * Angular rock. * Unsorted. * Unstratified.
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Based on restriction maps of plasmid determine the number of DNA fragments and sizes of the fragments?
Plasmids are circular pieces of DNA, so the number of fragments equals the number of cuts from the restriction enzymes. You can easily see this if you start with one restriction enzyme that cuts the plasmid in only one place. Cutting the circle in one place yields you only one fragment. If the restriction cuts in two places, you end up with two fragments; with three places, three fragments, etc. With linear chromosomes, the situation is different. Cutting a linear chromosome in one place yields two fragments, cutting in two places yields three fragments, etc. So the number of fragments is always one more than the number of cuts. A restriction map of a plasmid will show all of the cuts the restriction enzymes made. Each cut is labeled with the enzyme that made it. One can count the spaces between cuts to determine the number of fragments that are produced. Restriction maps usually (but not always) also show the size of each fragment.
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