this is the reverse of enzyme induction.here,E.Coli requires a regular supply of the amino acid tryptophan which is normally synthesized from raw materials using the enzyme tryptophan synthetase.if tryptophanis present in the growth medium, the bacteria will stop synthesizing tryptophan synthetase . Here, tryptophan is referred to as a corepressor.
Tryptophan will combine with the repressor molecule which was originally inactive converting it to an active repressor molecule which will be able to combine with the operator gene, this will make the gene to be repressed or switched off, it will not stimulate the structural genes so no mRNA will be transcribed therefore no enzyme will be produced.
When tryptophan is absent, the repressor molecule from the regulator gene remains in the inactive formand inthis form, it is unable to combine with the operator gene. So the operator gene remains switched on and therefore stimulates the structural gene,mRNA is then transcribed or for the production of mRNA. mRNA leads to the synthesis of a polypeptide which will lead to the productionof tryptophan synthetase
When an enzyme is heated it is denatured, which means that it can no longer function.
enzyme works as a catalyst before and after the reaction it is preserved
The enzyme gets denatured or inactive.
RNA polymerase is the enzyme that binds to DNA during transcription.
The enzyme is liberated free to repeat the action again. That is the beauty of enzymes.
nothing.
After a biochemical reaction, an enzyme typically remains unchanged and can be reused in other reactions.
During an enzyme-controlled reaction, enzymes act as catalysts to speed up the reaction without being consumed in the process. They bind to substrate molecules at their active sites, forming an enzyme-substrate complex that facilitates the conversion of substrates into products. Once the reaction is complete, the enzyme is released unchanged, ready to catalyze additional reactions. This allows enzymes to be reused multiple times in biochemical processes.
The enzyme still remains and can be used again for another reaction.
The enzyme responsible for reading DNA during transcription is called RNA polymerase.
Affinity decreases as the enzyme's geometry is modified by being denatured. It will no longer properly fit the active site.
The enzyme that breaks the hydrogen bonds during DNA replication is called helicase.