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You don't get a counterstain.
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∙ 15y ago
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What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What color will gram stain positive turn into?
If using a gram stain, they will turn Crystal Violet.
Why do you add crystal violet stain in simple staining method?
Because violet dye is the least expensive.
What color is gram negative stain and gram positive stain if you forget to apply iodine?
If iodine is not applied, both the gram-positive and gram-negative stains will appear to be gram-negative. The iodine acts as a mordant that helps to fix the crystal violet stain in the gram-positive bacteria, making them appear purple. Without iodine, the crystal violet stain can be easily washed out of both gram-positive and gram-negative bacteria, resulting in a pink or red color.
What would be the appearance of the gram positive bacterium if you forget to counterstain with safranin?
If you forget to counter stain color of Gram positive would be violet or blue . The above answer is good. Here is why the above answer is good. Yes it would still be Violet or blue. Gram positive bacteria are gram positive, because it holds onto the crystal violet stain that washes out of gram negative bacteria. Counterstaining with safranian turns gram negative bacteria pink to red only because the crystal violet has washed out of the gram negative. The lighter safranian has little to no effect on gram positive bacteria. The cause of the difference has to do with the makeup of the cell wall in the different bacteria.
What is the secondary stain in gram stain procedure?
It is crystal violet & stains all cells purple.
What color will gram stain positive turn into?
If using a gram stain, they will turn Crystal Violet.
Why do you add crystal violet stain in simple staining method?
Because violet dye is the least expensive.
What is the purpose of the crystal violet in the gram stain procedure?
Its the primary stain of the procedure. IT stains the Gram positive organisms
What is the purpose of crystal violet in the Gram's stain procedure?
Its the primary stain of the procedure. IT stains the Gram positive organisms
What is crystal violet iodine complex?
In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .
What color is gram negative stain and gram positive stain if you forget to apply iodine?
If iodine is not applied, both the gram-positive and gram-negative stains will appear to be gram-negative. The iodine acts as a mordant that helps to fix the crystal violet stain in the gram-positive bacteria, making them appear purple. Without iodine, the crystal violet stain can be easily washed out of both gram-positive and gram-negative bacteria, resulting in a pink or red color.
Why add saffranin?
Saffranin is a " counter stain " . this is also a " basic dye " which is in different color than primary stain ( basic dye crystal violet ) . The purpose counter stain ( saffranin ) is to give the decolourised cells a color , that is different from first one ( crystal violet ). The microbes which are not decolourised by alcohol retains the stain, while the decolourised cell take up the counter stain ( saffranin ).
What function of iodine in gram stain?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
Function of crystal violet?
Crystal violet is a hexamethyl also known as methyl violet 10B This is much darker than 2B, and often darker than 6B. It is used in biological stainang particularly gram staining together with safranin and iodine. Crystal violet was also used to treat strept throat. Doctors used to swab it on the back of your throat.
What is the action of iodine in grams stain?
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
What structure is stained in a counterstain?
Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with. If the violet can be washed out and the counter stain (pink) is added, the bacteria are Gram-.
