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Why normal DNA polmerase not use in PCR?
In the PCR, high temperatures are used in order to separate both strands of DNA readily. Normal DNA polymerases would "melt" (denature) under these conditions, whereas Taq DNA Polymerase does not (short from Thermus aquaticus, a bacteria that lives in very hot submarine springs).
Which molecule is responsible for separating double-stranded DNA into single strands?
At high temperatures, DNA denatures into single strands. A temperature around 95 degrees should work.
What happens in meiosis I that does not occur in meiosis I I?
DNA is copied.
What happens during S phase of the cell cycle?
This is the phase in which DNA is replicated.
How did Taq DNA polymerase acquire its name?
The Taq name is a shortened for Thermophilus aquaticus, a thermophilic bacteria that is the source of the particular DNA polymerase enzyme. The enzyme heat resistant property is desired because it could withstand the high temperature during the PCR process. -Kaitlin The Taq name is a shortened for Thermophilus aquaticus, a thermophilic bacteria that is the source of the particular DNA polymerase enzyme. The enzyme heat resistant property is desired because it could withstand the high temperature during the PCR process. -Kaitlin
Kary Mullis discovered a type of DNA polymerase that could withstand high temperatures Why did scientists want to heat up DNA replication?
This is because of Polymerase Chain Reaction (PCR). Basically, the problem is that you have a mixture of DNA, polymerase, primers etc, and you want to denature the DNA (separate both chains) - the denaturation happens at 94°C. Since the polymerase is present in the mixture, it has to withstand such temperature.
In DNA molecules Which bonds are disrupted at high temperatures?
hydrogen bonds
Could mammal polymerase be used to do pcr reactions?
Mammalian polymerase is heat labile. Which means, it denatures (or breaks up into fragments) at higher temperatures. Since PCR is a reaction what requires a high temperature for the DNA strands to denature, it would be more efficient to use a polymerase that could function at higher temperatures.
Can bacteria live in high heat?
How is it that some bacteria live in the hot springs of Yellowstone Park at temperature up as high as 73 C? Extreme thermophiles have adapted unique ways to surviving harsh conditions. They're enzymes are specially designed to resist denaturing at high temperatures. What's really amazing is their DNA. Extremophile DNA is positively supercoiled (this describes the way it is twisted around itself) which makes it harder to separate the strands. DNA in most other species is negatively supercoiled (meaning it's wound up on itself in the opposite direction as positively supercoiled DNA). Since DNA is the building block of life, it makes sense that you need to be able to protect it from the environment. Extremophiles do just that by positively supercoiling it. It is much more resistant to heat denaturing than other DNA would be, which helps the bacteria to survive.
Why do researchers us DNA polymerase from bacterium found in superheated water for PCR?
because they are more durable in high temperatures. Increasing the temperature is a way to increase the enzyme's production rate. Normally, a protein will denature at high temperatures. This way you can have the best of both worlds.
What does heat do to the DNA polymerase?
Heat denatures protein. DNA polymerase is an enzyme and a protein.
What is the role of heat in DNA extraction?
Heat anneals DNA strand i.e. separate two strands of DNA to build anti-codon to desired DNA strand
Why normal DNA polmerase not use in PCR?
In the PCR, high temperatures are used in order to separate both strands of DNA readily. Normal DNA polymerases would "melt" (denature) under these conditions, whereas Taq DNA Polymerase does not (short from Thermus aquaticus, a bacteria that lives in very hot submarine springs).
What can DNA do?
DNA can determine if a person will have a heat attack to if you will be good at snowboarding
Which molecule is responsible for separating double-stranded DNA into single strands?
At high temperatures, DNA denatures into single strands. A temperature around 95 degrees should work.
What happens during the S phrase?
During the S stage DNA synthesis occurs. (copying of the DNA)
What happens when there is a letter change in DNA?
what happen when dna letters are changed
