0
In the PCR, high temperatures are used in order to separate both strands of DNA readily. Normal DNA polymerases would "melt" (denature) under these conditions, whereas Taq DNA Polymerase does not (short from Thermus aquaticus, a bacteria that lives in very hot submarine springs).
Wiki User
∙ 12y ago
Add your answer:
Earn +20 pts
How can environmental scientist use DNA technology?
by using pcr to analyze DNA at a crime scene
Why was it difficult to use DNA as evidence in a crime before PCR was invented?
you need many copies of DNA for DNA fingerprinting
What does pcr stand for in biology terms?
The term pcr in biology stands for polymerase chain reaction. It stands for a process that biologists use in DNA sequencing, allowing them to make DNA copies more efficiently.
What is a Taqman Real Time PCR?
One can use a PCR to amplify and quantify a certain DNA molecule. A TaqMan real-time PCR is a certain type of PCR which uses the TaqMan method to increase its specificity.
Is Gel Electrophoresis used to make many copies of DNA?
no, it is used to separate different sized pieces of DNA using a gel and an electric current. Polymerase Chain Reaction (PCR) is the multiplication of DNA with the use of a PCR machine, enzymes and primers. The PCR machine allows the multiplication of DNA through temperature changes, activating each step of the reaction and copying DNA millions of times.
How can environmental scientist use DNA technology?
by using pcr to analyze DNA at a crime scene
Why was it difficult to use DNA as evidence in a crime pcr was invented?
you need many copies of DNA for DNA fingerprinting
Why was it difficult to use DNA as evidence in a crime before PCR was inventes?
you need many copies of DNA for DNA fingerprinting
Why was it difficult to use DNA as evidence in a crime before PCR was invented?
you need many copies of DNA for DNA fingerprinting
What process could you use to amplify the DNA in a bloodstain from a crime scene?
PCR
Is polymerase chain reaction used to amplify DNA from a fossil a fetal cell or a virus?
The PCR reaction can be used to amplify DNA from all three sources mentioned. PCR relies on the use of short stretches of DNA that are 6 - 12 bases long to attach to the target DNA (the source where the DNA is coming from) so that the polymerase enzyme can make copies of the target DNA. As long as these primers are available (they can be commercially purchased in many cases), PCR can be carries out on fetal cell DNA and viral DNA. Fossil DNA however, may have undergone degradation. DNA has to be of a certain purity for PCR to work. If the fossil DNA had degraded or broken down, PCR cannot be carried out.
What does pcr stand for in biology terms?
The term pcr in biology stands for polymerase chain reaction. It stands for a process that biologists use in DNA sequencing, allowing them to make DNA copies more efficiently.
What is a Taqman Real Time PCR?
One can use a PCR to amplify and quantify a certain DNA molecule. A TaqMan real-time PCR is a certain type of PCR which uses the TaqMan method to increase its specificity.
Is Gel Electrophoresis used to make many copies of DNA?
no, it is used to separate different sized pieces of DNA using a gel and an electric current. Polymerase Chain Reaction (PCR) is the multiplication of DNA with the use of a PCR machine, enzymes and primers. The PCR machine allows the multiplication of DNA through temperature changes, activating each step of the reaction and copying DNA millions of times.
What is the correct sequence of events that occur in a PCR reaction?
separation of DNA strands; addition of primers; use of DNA polymerase to produce second strand of DNA
What is the purpose of mgcl in pcr?
The enzyme DNA polymerase ( Taq polymerase) used in the PCR requires Mg 2+ ions for its functioning.These Ions act as cofactors for the enzyme . Hence the requirement for the use of Mg Cl2 in PCR reactions.
What reagents do you use in pcr?
in pcr technique we take original dna first heat it to separate to strands in thermocycler then add rna primer after the formation of about 10 sequences on both parental strands add dna polymerase to construct further
