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This is because of Polymerase Chain Reaction (PCR). Basically, the problem is that you have a mixture of DNA, polymerase, primers etc, and you want to denature the DNA (separate both chains) - the denaturation happens at 94°C. Since the polymerase is present in the mixture, it has to withstand such temperature.

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Q: Kary Mullis discovered a type of DNA polymerase that could withstand high temperatures Why did scientists want to heat up DNA replication?
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