Want this question answered?
mannitol is a type of sugar, so it supplies the carbon in the MSA medium
Ingredients Gms / Litre Glucose 20.000 Dipotassium phosphate 0.200 Magnesium sulphate 0.200 Sodium chloride 0.200 Potassium sulphate 0.100 Calcium carbonate 5.000 Agar 15.000 Final pH ( at 25°C) 7.4±0.2
how to make a well on the agar for pour the extract
dear agar mujhe pata hota to pagal kutte ne kata tha ki mai ise google par search karta
The gel in a Petri dish is called "Agar".
There are many ingredients in PEA that supply carbon. Some of the ingredients are dextrose, yeast extract, animal tissue, and soy peptone.
pancreatic digest of casein
Casein is the ingredient in PEA that supplies carbon, as well as nitrogen. PEA is also known as Phenylethyl Alcohol Blood Agar.
Different nutrient agars have different ingredients in them. Agar itself is a polysaccharide (sugar) composed of galactose. Most agars contain some sort of soybean or lipids and also amino acids. Many contain peptone, which is a mixture of polysaccharides and protein.
The key nutrient ingredients required when preparing a culture medium for bacteria growth are a carbon source (like glucose), a nitrogen source (like peptone or ammonium salts), minerals (such as magnesium and potassium), vitamins, and water. Additional ingredients, such as agar for solidification and pH indicators, may also be included depending on the specific needs of the bacteria being cultured.
the east extract is the main source of nitrogen in the nutrient broth or agar
mannitol is a type of sugar, so it supplies the carbon in the MSA medium
what foods contain agar
PEA agar, also referred to as Phenylethyl Alcohol Agar is a medium used by microbiologists to grow microorganisms. The ingredient that supplies nitrogen is casein.
Pancreatic digest of casein, papaic digest of soybean meal, beef extract, peptone
Agar or agar-agar is gelatin substances that can be obtain from algae. In culinary agar refers to a vegetable gelatin that is being used to make jellies, puddings, and custards products.
sabourauds agar contains the following : peptone : 1 g glucose : 4 g agar :3 g d/w : 100ml PH : 5.4