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What is CsCl profile of a DNA and why CsCl is used for ultracentrifugation?
Wiki User
∙ 13y ago
Since CsCl is a kind of heavy salt, it forms density gradients when centrifugation. When sedimentation equilibrium, DNA moves to the position where meets its density. The density of DNA is related to its GC%, by analytical ultracentrifugation, we can read the GC levels of the DNA from its position in the solution.
Please refer to the article for detail:
Using analytical ultracentrifugation to study compositional variation in vertebrate genomes (Euro Biophys J. 2003 32: pp418-426)
Wiki User
∙ 13y ago
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How many markers are used to make a dna profile?
2
How many cells are needed to profile DNA?
10
What pieces of evidence would be considered the best for establishing biological relatedness?
a very close match in the DNA profile
Why DNA is so useful in forensics and identification?
D.N.A. is used to help solve crimes by the fingerprint in the DNA which can track down the person at the scene of the crime. Also, if a sample of the body is left (i.e. blood, any other bodily fluids, hair, skin) is left at the scene, through DNA finger printing, the criminal(s) involved would be identified.
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Hair can be used for a DNA profile.
How many markers are used to make a dna profile?
2
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How many cells are needed to profile DNA?
10
What is DNA profile?
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Did the US develop a DNA Profile for Osama bin Laden?
No
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What federal agency is responsible for managing the database of DNA profile?
FBI
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What is the role of Cscl and ethidium bromide in plasmid purification?
The CsCl forms a gradient and the molecules migrate according to their density until they float at their individual isopycnic points (the point in the gradient that equals the buoyant density of the molecule). However, plasmid DNA and contaminating chromosome have about the same density and cannot be separated easily. This is rectified, however, by the addition of ethidium bromide. Density is a function of AT/GC ratio, but it is also a function of conformation. For supercoiled DNA, there is more DNA per unit volume than for relaxed DNA. Intercalation of ethidium bromide into DNA causes the helix to unwind (negative supercoiling) and become more relaxed. However, negative supercoiling only relaxes the DNA to a point. Further unwinding induces supercoiling in the opposite direction. When the DNA is circular and the ends are connected, the plasmid "kinks up" into a very tight knot. Thus, ethidium bromide causes the plasmid density to be increased.
How is a killer identified when a fingerprint has never been taken of them?
DNA profiling is a technique employed by forensic scientists to assist in the identification of individuals on the basis of their respective DNA profiles. To identify individuals, forensic scientists scan 13 DNA regions, or loci, that vary from person to person and use the data to create a DNA profile of that individual. There is an extremely small chance that another person has the same DNA profile for a particular set of 13 regions.
