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DNA polymerase III (not DNA polymerase) is an enzyme that works in association with other enzymes during the replication of a DNA molecule. DNA replication begins when the enzyme, known as helicase unwinds a DNA strand. Helicase unwinds a DNA strand, thus, in the process, separating the two DNA templates. The result of the unwinding of the DNA molecule is the formation of a replication bubble. Once a DNA molecule is unwound, it is not stable. The DNA molecule is untwisted, broken and rearranged by an enzyme called topoisomerase in order to create stability at the ends of a replication bubble. In addition, the DNA replication bubble is further stabilized by a group of protein complexes known as single strand binding proteins.
Once the DNA molecule is unwound and stabilized, an enzyme called primase assembles an RNA sequence that is complementary to the adjacent DNA template. The purpose of this initial RNA sequence is to provide a point at which DNA polymerase III can start to add nucleotides to the corresponding DNA template. Unlike RNA polymerase, DNA polymerase III requires an RNA sequence, which is known as a primer. DNA polymerase III can attach a nucleotide only to the 3 prime end of an existing nucleotide sequence. Once a primer is assembled by primase, DNA polymerase III begins its work of adding nucleotides to the 3 prime end of the primer.
It is important to note that replication proceeds in two directions, since a DNA replication bubble consists of two DNA templates. Since DNA polymerase III proceeds in the three prime to 5 prime direction at one DNA template, it also has to proceed in the 3 prime to 5 prime direction on the other DNA template. Since the template run in opposite directions, the second template will consist of multiple primers and thus short segments of DNA. These short segments of DNA are known as Okazaki fragments. The Okazaki fragments are created by DNA polymerase three since it is only able to proceed in the 3 prime to 5 prime direction.
After DNA polymerase III completes its work, DNA polymerase I begins to replace the RNA nucleotides of the primers with DNA nucleotides. Once DNA polymerase I replaces the RNA nucleotides with DNA nucleotides, DNA ligase joins the Okazaki fragments together and the result is a new DNA template.
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What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
What enzymes are responsible for replication of the DNA molecule?
Helicase is the enzymes that splits the double helix into two separate strands, and DNA Polymerase (as opposed to RNA Polymerase) joins the nucleotides together in the new strands being created.
What enzyme binds to DNA and is responsible for making a transcript RNA of DNA?
RNA polymerase is the enzyme responsible for binding to DNA and synthesizing a complementary RNA strand during transcription.
Can DNA polymerase unwind DNA?
No, DNA polymerase cannot unwind DNA. DNA polymerase is responsible for synthesizing new DNA strands by adding nucleotides to a template DNA strand. The unwinding of DNA is typically performed by DNA helicase enzymes.
What class of enzyme is DNA polymerase?
DNA polymerase is a catalyst, by catalyzing the synthesis of new DNA by adding nucleotides to a preexisting chain. There are several different DNA polymerases, but DNA polymerase I and DNA polymerase II play the major roles in DNA replication.
What chemical is an enzyme that add nucleotides to a new strand of DNA during replication?
DNA Polymerase is the enzyme which adds new nucleotides during replication.
Is the enzyme that allows replication to occur DNA polymerase or RNA polymerase?
DNA polymerase replicated DNA. RNA polymerase creates mRNA to be used in protein synthesis. RNA polymerase does not replicated DNA.
Is DNA polymerase a protein?
Yes, DNA polymerase is a protein.
What enzyme transcribes DNA?
The enzyme that transcribes the DNA into RNA is called RNA polymerase.
What are the key differences between DNA polymerase and RNA polymerase in terms of their functions and roles in the process of genetic information replication?
DNA polymerase is responsible for synthesizing new DNA strands during DNA replication, while RNA polymerase is responsible for transcribing DNA into RNA. DNA polymerase adds nucleotides to the growing DNA strand, ensuring accurate replication of genetic information. RNA polymerase reads the DNA template and synthesizes a complementary RNA strand. Overall, DNA polymerase is involved in DNA replication, while RNA polymerase is involved in transcription.
What is the name of the enzyme that match the DNA bases?
DNA polymerase matches the bases on the parent strand.
How does DNA polymerase function in the process of transcription?
DNA polymerase does not function in the process of transcription. Transcription is the process where RNA is synthesized from a DNA template by RNA polymerase. DNA polymerase, on the other hand, is involved in DNA replication, where it synthesizes a new DNA strand using a DNA template.
What is the name of the two enzymes that help in the duplication of DNA?
More than two enzymes are involved. However, the main ones are DNA Polymerase I and DNA Polymerase III. DNA Polymerase III adds new nucleotides and DNA Polymerase I removes primers.
Is DNA polymerase used in the process of transcription?
No, DNA polymerase is not used in the process of transcription. Transcription is the process of making an RNA copy of a gene from DNA, and it is carried out by an enzyme called RNA polymerase. DNA polymerase is primarily involved in the process of DNA replication.
Enzyme used to position nucleotides during DNA replication?
DNA polymerase is the enzyme responsible for positioning nucleotides during DNA replication. DNA polymerase can add nucleotides to the growing DNA strand in the 5' to 3' direction and proofread for errors in base pairing.
What enzymes are responsible for replication of the DNA molecule?
Helicase is the enzymes that splits the double helix into two separate strands, and DNA Polymerase (as opposed to RNA Polymerase) joins the nucleotides together in the new strands being created.
What does heat do to the DNA polymerase?
Heat denatures protein. DNA polymerase is an enzyme and a protein.
