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Q: What is LB plate in making competent cells?
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Which plates should be compared to prove that the changes that occurred were due to the procedure that you performed?

The best way to prove that these changes occurred in the transformation lab is to compare the control to the experimental plates. Cells that were not treated with the plasmit (LB/amp (-) pGLO and LB/amp/are (-) pGLO plates) could not grow on ampicillin, wheras cells that were treated with the plasmid (LB/amp (=) pGLO and lB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. Thus, the plasmid must confer resistance to ampicillin.


How would you change the bacterias plate to best tell if they are ampicillin resistant?

The best test would be to take some of the bacteria growing on the LB plate and streak them on a LB/amp plate. If the bacteria are viable on the LB/amp plate, then they are resistant to ampicillin. If no bacterial colonies survive, then they were not ampicillin resistant.


Can you tell if these bcteria are ampicillin resistant by looking at them on the LB plate?

No. You cannot tell if the bacteria are ampicillin resistant just by looking at them. Both types of bacteria (those that are ampicillin resistant and those that are ampicillin sensitive) look similar when cultured, think about the colonies on the LB starter plate and the colonies on the +pGLO LB/amp plate.


Can DH5alfa cells grow on lb agar plates?

Yes.


Which cells secrete the antibodies?

Lymphocytes LB


How much are the registration and license plate fee in Illinois for a 33000 lb truck?

$293


What are the torque specs for Honda flywheel and pressure plate?

I believe that these specs do vary from model to model, but here are the specs for a 97-01 Prelude. Pressure plate bolts - 19 lb ft Flywheel bolts - 76 lb ft


On which plate would you expect to find bacteria most like the original non transformed E. coli colonies you initially observed?

You would expect to find it in the plate labeled LB-, because that specific plate is the control plate, meaning it has nothing added to it, like the amipicilin or pFlouroGreen plasmid.


What are torque specs for 94 Nissan 300zx pressure plate?

25-33 ft-lb


What are the torque specs for a 2004 Yamaha blaster outer pressure plate for the clutch springs?

Yamaha Blaster Inner Clutch Spring Torque(5 Bolts with Springs) 4.3Ft-Lb. (51.6In-Lb.) Also, Center Bolt 5.1Ft-Lb. (61.2In-Lb.)


Can capsicum powder can be done at home?

making your child obese in 120 lb


What does LB stand for in LB agar?

LB stands for Luria Bertani. It is a growth medium used for most of the bacterial cultures, compositions of LB broth is 10g of tryptone, 5g of yeast extract and 10g of NaCl. For LB medium add 75g/l of Bacteriological buffer in the broth and pour it in the plate to solidify,.