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How would you change the bacterias plate to best tell if they are ampicillin resistant?
Wiki User
∙ 11y ago
The best test would be to take some of the bacteria growing on the LB plate and streak them on a LB/amp plate. If the bacteria are viable on the LB/amp plate, then they are resistant to ampicillin. If no bacterial colonies survive, then they were not ampicillin resistant.
Wiki User
∙ 11y ago
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Can you tell if these bcteria are ampicillin resistant by looking at them on the LB plate?
No. You cannot tell if the bacteria are ampicillin resistant just by looking at them. Both types of bacteria (those that are ampicillin resistant and those that are ampicillin sensitive) look similar when cultured, think about the colonies on the LB starter plate and the colonies on the +pGLO LB/amp plate.
What is the purpose of a control agar plate?
A control in an agar plate is the left over spot where you have put no bacteria swabs on, that way when the bacterias have grown you can see if the bacterias spread onto the control, or if the control stayed disinfected.
What is meant by a control plate in pglo experiments?
a control plate in your particular case is a ampicillin plate with no inoculation of your transformed bacteria. its purpose to make sure that during the process of handling the plate there were no contamination by bacteria in the surrounding.
Are there any shatter resistant plate sets?
Behrenberg Company makes a shatter resistance plate set.
What if a agar plate is isolated and antibiotic added and result is no growth or inhibition?
This is basically ANTIBIOTIC SENSITIVITY TEST, to test whether the given organism is RESISTANT(no zone of inhibition) or SENSITIVE( zone of inhibition) to the given antibiotic.Zone of Inhibition Testing is a fast, qualitative means to measure the ability of an antimicrobial agent to inhibit the growth of microorganisms.The effectiveness is based upon the size of zone of inhibition,diffusability of antibiotic,size of inoculum,type of media used.example: bacillus organism is inoculated with both PENICILLIN and AMPICILLIN ,zone of inhibition is absent in case of penicillin and present in case of ampicillin, this shows that ampicillin (sensitive) worked effectively when compared to penicillin.
Is breaking a plate a chemical change?
No breaking a plate is a physical change. A chemical change is a change to a substance where its identity changes. When you break a plate you still have a plate not a new substance.
Is a plate is dropped and shattered a chemical change?
A plate dropping and shattering is a physical change. It is not a chemical change, as the material used in making the plate doesn't change.
How do antibiotic resistant bacteria come about?
This term is misleading. The antibiotic "selects" bacteria that are not affected by it. If a person will grow bacteria on a petri dish and add an antibiotic to it, some bacteria may live and grow. This is actually a form of natural selection. The ones that will grow are resistance to the antibiotic. They have some way of not being affected. If a person takes a colony from the plate that has this resistance and grows it on another plate and add the antibiotic, all on the plate will be resistant.
On which plate would you expect to find bacteria most like the original non transformed E. coli colonies you initially observed?
You would expect to find it in the plate labeled LB-, because that specific plate is the control plate, meaning it has nothing added to it, like the amipicilin or pFlouroGreen plasmid.
How do you remove skid plate to change oil filter on a Toyota Sequoia?
The skid plate does not have to be removed in order to access and change the filter.
Which plates should be compared to prove that the changes that occurred were due to the procedure that you performed?
The best way to prove that these changes occurred in the transformation lab is to compare the control to the experimental plates. Cells that were not treated with the plasmit (LB/amp (-) pGLO and LB/amp/are (-) pGLO plates) could not grow on ampicillin, wheras cells that were treated with the plasmid (LB/amp (=) pGLO and lB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. Thus, the plasmid must confer resistance to ampicillin.
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