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Rabbit plasma, sometimes with EDTA added
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What is difference between tube coagulase and slide coagulase test?
tube coagulase is done in test tube while slide coagulase is done on slide.thnk you all! cheeres!
Test used for the identification of Staphylococcus aureus?
staph. aureus test positive for catalase test and coagulase test consecutively
Is coagulase test a microbiological test?
YES
Is citrate test results for Staphylococcus aureus?
No, Catalase +ve, Coagulase +ve, Gram + cocci. Citrate test is usually used to differentiate members of the Family Enterobacteriaceae. Check Bergeys systematic bacteriology. 1980
What are the general characteristics of staphylococcus Aureus?
Gram + coccus. Positive methanol test. Yellow on agar.
What is difference between tube coagulase and slide coagulase test?
tube coagulase is done in test tube while slide coagulase is done on slide.thnk you all! cheeres!
Why is it important to use fresh cultures in a coagulase test?
Coagulase is a protein enzyme. It is important to use fresh cultures in a coagulase test because coagulase expression is highest during the log phase of growth.
Test used for the identification of Staphylococcus aureus?
staph. aureus test positive for catalase test and coagulase test consecutively
Is coagulase test a microbiological test?
YES
What is bound coagulase?
Coagulase is a protein produced by several organisms, namely Staphylococcus and in laboratories is used to distinguish different isolates.Bound coagulase is part of the larger family of MSCRAMM.
Is citrate test results for Staphylococcus aureus?
No, Catalase +ve, Coagulase +ve, Gram + cocci. Citrate test is usually used to differentiate members of the Family Enterobacteriaceae. Check Bergeys systematic bacteriology. 1980
What is a free coagulase and its significance?
Free coagulase is an extracellular toxin that reacts with a thrombin-like, thermostable substance known as coagulase-reacting factor (CRF) or serum factor. The reaction of coagulase and CRF will lead to the formation of coagulase-CRF complex where this complex indirectly changes frbrinogen to fibrin (clotting factor) and hence will form a fibrin clot. Furthermore, it is also use as the primary indicator of virulence among staphylococci. Its clinical significance is that when doing the coagulase tube test, the free coagulase will react with the plasma in the tube and hence convert the fribrinogen into fibrin which lead to fibrin clot formation in the tube. This is shown after one day of incubation of the tube, when the tube being invert, the plasma will not go down showing that it is clotted.
What is the principle behind the coagulase test?
in the coagulase test, normally a virulent staphylococcus is put into plasma. plasma contains the plasma protein fibrinogen. when fibrinogen is acted upon by a fibrinogenase, it is then converted to fibrin. fibrin has te ability to form clots. so in the case of staphylococcus aureus which has the enzyme coagulase also called fibrinogenase, the enzyme acts on the fibrinogen in plasma which is converted to fibrin hence the clots that are seen suspending in the solution after 4 hours.
What are 2 types of staphylococal coagulase?
2 types of staphylococal coagulase are 1.staph.epidermidis/albus2.staph.aureus
What are the general characteristics of staphylococcus Aureus?
Gram + coccus. Positive methanol test. Yellow on agar.
What is a CNS and what do they do?
coagulase-negative staphylococci (CNS)
What is the carbohydrate profile for S epidermidis?
coagulase
