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What happens after genetically modified plasmids inserted into bacteria?
Plasmids are cloned and start producing protein .
What is genetic engineering in animals?
GE is removing DNA from selected animals then adding new genes (which posses different traits) into the DNA itself before implanting the cell into the host
What is a vector used for in a genetic engineering experiment?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".
What is the methodology for producing recombinant DNA to be used in gene cloning?
1. A vector such as plasmid is needed along with a host cell. Restriction enzymes and DNA ligase are enzymes that are used to introduce foreign DNA into a vector.
What are the steps of using recombinant DNA technology?
1 Isolate DNA 2 Cut DNA with a restriction enzyme 3 Mix the DNA's and join then together by using DNA ligase 4 Insert the recombinant plasmid into a host bacterium 5 Allow the bacterium to reproduce
Why do viruses depend in host cells?
Viruses are composed of protein and DNA. The DNA encodes the protein as well as the DNA for the virus. Viruses depend on host cells because they are incapable of reproducing themselves. They enter the host cell and the viral DNA is inserted into the host DNA. The virus then "hijacks" the host cells replication machinery to make more viral protein and viral DNA.
What is the role of restriction enzymes in bacteria?
Restriction enzyme in bacteria cuts of the foreign DNA inside the host, thus destroying them. The host DNA is protected against this destructive action due to methylation of the host DNA.
An infection in which DNA of a virus is embedded into a host cell and replicates with host DNA is called what?
A Lysogenic Infection
What is the viral DNA that has attached to a host cell's chromosomes and that is replicated with the chromosome's DNA?
Many viruses can infiltrate a host's DNA to replicate itself. An example of this is the AIDS virus.
What enzyme inserts viral DNA into the host's chromosomal DNA?
integrase
What engineering involves inserting foreign dna into host dna to make recombinant DNA?
genetic
The viral reproductive cycle in which a phage injects its DNA into a host cell and the DNA is inserted into the host cell's chromosome is called the?
lysogenic
What is plus DNA and minus DNA?
If we are talking about viruses and their RNA genomes(eg:HIV), the negative strand DNA synthesis by reverse transcriptase occurs in the host cells, when the virus infects the host. And then, plus DNA formed by complementing this minus DNA.
Where does a virus replicate its DNA?
A virus replicates its DNA in a cell when it infects the host
The viral reproductive cycle in which a phage injects its DNA into a host cell and the DNA is inserted into the host cells chromosome is called the?
motor cycle
Do Most DNA viruses multiply in the host cell's cell membrane?
No, DNA viruses multiply in the host cell's nucleus, while most RNA viruses multiply in the host cell's cytoplasm
The viral reproductive cycle in which a phage injects its DNA into a host cell and the DNA is inserted into the host cell's chromosome is called the cycle?
B) Lysogenic
