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Why chilled ethanol is added for DNA isolation?
to precipitate extracted DNA
What is the purpose of the modified bases in DNA sequencing?
- DNA replication terminates when a modified base is added. - They are easily recognized by the computer. - They are added to the DNA chain just like the normal bases.
What is the purpose of DNA ligase?
It is an enzyme that is added to help bond the ends of DNA fragments together.
Why is isopropanol used to precipitate DNA and what does it do to DNA to make it precipitate?
As DNA is completely soluble in water, but not in alcohol, like isopropanol, when isoprop is added, its engaged more and more water molecule to interact, as a result, less water molecules are available to dissolve DNA, and DNA statrs ppt out.
When the wrong nucleotides are added to a strand of DNA during DNA replication they can never be repaired?
Plato .... False
Which enzyme is added to attach the DNA backbone between vetor and foreign DNA that have formed bases pairs at sticky ends during of recombinant DNA?
DNA ligase is added.
What enzyme is added to attach the DNA backbone between vector and foreign DNA?
DNA ligase
Why chilled ethanol is added for DNA isolation?
to precipitate extracted DNA
What enzyme called is added to attach the DNA backbone between vector and foreign DNA?
DNA ligase
Why rna virus is more mutant than dna virus?
during replication RNA-polimeraze it make a lot of erros.In this ways RNA viruses it mutate faster than DNa viruses.
In regards to analyzing DNA with gel electrophoresis Are DNA fragments separated by size?
Yes, electrophoresis involves seperation depending upon size by applying charge to the DNA sample loaded which then travels form negative to positive eletrode as DNA being negatively charged. Thus the small sized molecules will travel faster as compared to larger molecules.
What is the purpose of the modified bases in DNA sequencing?
- DNA replication terminates when a modified base is added. - They are easily recognized by the computer. - They are added to the DNA chain just like the normal bases.
Where do fish get there glow?
Glofish are really Zebra Danios that have had a jellyfish gene added artificially to make them glow.
Two new strands of DNA molecules grow as bases are added by the enzyme?
DNA polymerase.
Function of agarose in agarose gel electrophoresis?
Agarose is used in gel electrophoresis to separate nucleic acids (like DNA) by size, charge an other physical properties. Gel electrophoresis uses an electrical current to make particles move. For example, DNA is negative, so it'll travel towards to positive electrode of the gel box. Agarose has small pores through which a DNA can travel. Bigger fragments of DNA travel shorter distances, because it takes longer for them to navigate through the pores of the agarose gel. Identically sized pieces of DNA will travel the same distance, which is why you get bands (DNA with loading dye) after you run a a gel.
What end of a growing strand are DNA nucleotides added to?
5' end (nucleotides are added from 3' toward 5')
What prevents the wrong nucleotide from being added to the new strand during DNA?
DNA Polymerases
