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Why you have the range of concentration of agarose in gel electrophoresis?
increasing the agarose concentration will enable the separation of smaller fragments of DNA. the structure of the gel (agarose) consists of crosslinks, therefore the higher the concentration of agarose the more crosslinks there will be and smaller size "holes" for the DNA to travel through (also the other way around, with less concentrated agarose)
Minimum DNA on agarose to visualize?
you need at least 20ng to visualize it on an agarose gel along with EtBr or GR safe
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
Why would a scientist use a higher versus a lower percent agarose solution when preparing a gel to separate DNA?
Different percentages have different resolving powers. There is no one agarose percentage that is suitable for all sizes of DNA - you must chose the percentage best for resolving the sizes of DNA you are examining. If your agarose concentration is too dense for the size of your DNA fragments, the DNA will barely migrate through the gel. If the agarose concentration is too dilute for the size of your DNA, it will run straight through the gel without resolving into sharp bands. Generally speaking you use higher percentages if you want to resolve smaller DNA fragments and lower percentages if you want to resolve larger DNA fragments. Small DNA fragments need high percentages or else they'd run straight through the gel without being resolved into bands. Large DNA fragments need low percentages to permit them to migrate into the gel.
What is an example of a gel?
example of gel is agarose gel,
High concentration agarose will?
impede migration
Why you have the range of concentration of agarose in gel electrophoresis?
increasing the agarose concentration will enable the separation of smaller fragments of DNA. the structure of the gel (agarose) consists of crosslinks, therefore the higher the concentration of agarose the more crosslinks there will be and smaller size "holes" for the DNA to travel through (also the other way around, with less concentrated agarose)
What is role of agarose of electrophoresis?
agarose helps in the separation of DNA bands by controlling the pore size of agarose gel
What is agarose and what is the TM value of agarose?
Agarose is a linear polysaccharide used for gel mediums. Tm (melting temp) is about 85 C.
What is the relationship between the size of the DNA fragment and the distance that it migrates from the well?
. Because DNA is a negatively charged molecule, it will migrate through the gel toward the positive electrode (recall that opposite charges attract). The rate of migration of DNA through the agarose depends on the size of the DNA fragment. The smaller the fragment, the faster it can move through the gel. Another important factor is the concentration of agarose in the gel. The higher the concentration of agarose, the more it slows down the movement of all the DNA fragments.
What is an agarose?
An agarose is a polymeric cross-linked polysaccharide extracted from the seaweed agar and used to make gels.
Minimum DNA on agarose to visualize?
you need at least 20ng to visualize it on an agarose gel along with EtBr or GR safe
Preparation of 1 percent agarose gel or How to prepare 1 percent agarose gel?
Check the answer for How do you make an electrophoresis gel?
Is agarose gel electrophoresis suitable for only gram negative plasmid DNA?
Agarose gel electrophoresis is suitable for ALL DNA.
What holds agarose gel together?
Stuff
Agarobiose?
is the repeating unit of the agarose polymer
Use of agrose in DNA isolation?
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
