Agarose is not used in DNA isolation.
Agarose is used to prepare a gel in which DNA fragments can be separated based on size
Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
it is SDS like detergent added at to lyse cells
CTAB is a detergent used to denature proteins from samples. Once the protens have been denatures, the isolation of DNA from the non required waste materials can begin.
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
The DNA fragments comes from the method of DNA isolation.
DNA isolation is a based on the principle of purification. DNA samples are isolated through the use of physical and chemical methods. Friedrich Miescher conducted the first isolation of DNA in 1869.
DNA is soluble in chloroform more than water. So we use it.
helps in DNA ppt
Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
it is SDS like detergent added at to lyse cells
CTAB is a detergent used to denature proteins from samples. Once the protens have been denatures, the isolation of DNA from the non required waste materials can begin.
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
it act as as a cationic detergent for the isolation dna from the given sample
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
The DNA fragments comes from the method of DNA isolation.
to precipitate extracted DNA
Sodium acetate buffer helps by reacting with the membrane protein and precipitating them, thus facilitating the dna isolation.