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Several DNA isolation protocols recommend the use of either ethyl or isoamyl alcohol for the precipitation step
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∙ 15y ago
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What is the function of 2-propanol in DNA extraction?
To achieve precipitation DNA.
What properties of DNA are responsible for its precipitation in alcohol?
because of the negative charge in alcohol and the dna. it makes the dna not stay together.
How does phenomena of solution and precipitation of DNA relate to its physical and chemical structure?
Precipitation is the result of a chemical reaction. This reaction can degrade the physical and chemical structures of DNA molecules, which will render them unviable.
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
What is the function in the following reagents used in the extraction and precipitation of DNA?
to extract the DNA from the solution, isopropyl alcohol is mixed with the DNA solution to precipitate the same.
What is the action of NaCl in DNA?
NaCl help the precipitation and separation of DNA.
What is the function of 2-propanol in DNA extraction?
To achieve precipitation DNA.
What is the role of NaCl in DNA precipitation?
roll of Na CL in DNA extraction
What is use of ethanol in DNA isolation?
Ethanol is used to precipitate the DNA. I.e. to bring the DNA out of solution. Precipitated DNA is then spun down and re suspended in the appropriate buffer that is suitable for sample storage
How does sodium chloride effects on DNA?
Sodium chloride help the precipitation and separation of DNA.
What properties of DNA are responsible for its precipitation in alcohol?
because of the negative charge in alcohol and the dna. it makes the dna not stay together.
How does phenomena of solution and precipitation of DNA relate to its physical and chemical structure?
Precipitation is the result of a chemical reaction. This reaction can degrade the physical and chemical structures of DNA molecules, which will render them unviable.
What is the purpose of Trichloroacetic acid in DNA extraction?
Trichloroacetic acid is used for precipitation of the DNA during its extraction.
What is the function in the following reagents used in the extraction and precipitation of DNA?
to extract the DNA from the solution, isopropyl alcohol is mixed with the DNA solution to precipitate the same.
How many volumes of isopropanol is to be used for alcohol precipitation of DNA?
0.6 volume
How do the phenomena of solution and precipitation of DNA relate to its physical and chemical structure?
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Why does addition of ice-cold ethanol lead to the precipitation of DNA?
Because it need to preserve for DNA expert to use. Just like all the living things if an animals is died in snow, snow are used to preserve them. Same as DNA
