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What is the ampr gene?
It is a gene which is present in vectors that code for an enzyme that cleaves ampicillin, which inhibits the ampicillin from preventing Save the growth of bacteria.
Which enzyme would cut the plasmid without disrupting the function of?
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
What is the purpose of the ampicillin in the transformation procedure?
The ampicillin resistance gene (AmpR) is called a selectable marker gene and is incorporated into several plasmids that are commonly used in a wide range of genetic engineering and molecular biology experiments. The function of a selectable marker gene is to provide the host containing the plasmid a certain property that is not inherently present in the host. For example, the AmpR gene codes for a protein that makes cells containing this gene resistant to the antibiotic ampicillin. Therefore, when plasmids are successfully transfected into bacterilal cultures, one can assess which colonies have taken up the plasmid by looking at which ones survive onampicillin-containing media. Those that do not survive do not have the plasmid. The surviving colonies can then be picked up and carried on to the next stage of experimental research.
How can bacteria develop an immunity to antibiotics?
the way bacteria are able to develop immunity through transformation is the take up plasmids, small loops of DNA ranging from 6 kb to 100 kb (kilobase pairs), from their surrounding environment, which in some cases has a gene for antibiotic immunity the most common one is Ampr, Ampicillin resistance
What is the ampr gene?
It is a gene which is present in vectors that code for an enzyme that cleaves ampicillin, which inhibits the ampicillin from preventing Save the growth of bacteria.
Which enzyme would cut the plasmid without disrupting the function of?
Perhaps you mean a restriction enzyme, but not disrupting the function of whatever is not too clear. I think if you cut a plasmid with any restriction enzyme I am familiar with the function of that plasmid would be disrupted.
What is the purpose of the ampicillin in the transformation procedure?
The ampicillin resistance gene (AmpR) is called a selectable marker gene and is incorporated into several plasmids that are commonly used in a wide range of genetic engineering and molecular biology experiments. The function of a selectable marker gene is to provide the host containing the plasmid a certain property that is not inherently present in the host. For example, the AmpR gene codes for a protein that makes cells containing this gene resistant to the antibiotic ampicillin. Therefore, when plasmids are successfully transfected into bacterilal cultures, one can assess which colonies have taken up the plasmid by looking at which ones survive onampicillin-containing media. Those that do not survive do not have the plasmid. The surviving colonies can then be picked up and carried on to the next stage of experimental research.
How can bacteria develop an immunity to antibiotics?
the way bacteria are able to develop immunity through transformation is the take up plasmids, small loops of DNA ranging from 6 kb to 100 kb (kilobase pairs), from their surrounding environment, which in some cases has a gene for antibiotic immunity the most common one is Ampr, Ampicillin resistance
